小鼠血小板活化因子(PAF)ELISA試劑盒子科生物研發(fā)生產(chǎn)現(xiàn)貨供應(yīng),深圳子科生物提供大量品牌進(jìn)口原裝,分裝,以及穩(wěn)定性強(qiáng)的自產(chǎn)國(guó)產(chǎn)人ELISA試劑盒,大鼠ELISA試劑盒,小鼠ELISA試劑盒,魚(yú)ELISA試劑盒,植物ELISA試劑盒,牛羊豬雞ELISA試劑盒性價(jià)比*。咨詢!
?產(chǎn)品名稱:小鼠血小板活化因子(PAF)ELISA試劑盒
?英文名稱:Mouse plaet activating factor,PAF ELISA KIT
?產(chǎn)品規(guī)格:96T(人份)/48T(人份)(單孔檢測(cè)分別可以檢測(cè)90份樣本,42分樣本,一般都需要留6個(gè)孔做標(biāo)準(zhǔn)曲線)。
?檢測(cè)方法:酶聯(lián)免疫分析ELISA方法
?有效期: 4 個(gè)月(4℃);8 個(gè)月(-20℃)。
?保存方法: 2-8℃(頻繁使用時(shí)); -20℃(長(zhǎng)時(shí)間不用時(shí))。
?品牌:子科生物ZIKER,美國(guó)R&D,美國(guó)immonoway,美國(guó)sciencell,德國(guó)IBL
?質(zhì)量保證:我告訴所有產(chǎn)品均需要經(jīng)過(guò)質(zhì)檢通過(guò)后,才允許出庫(kù)!
?適用范圍:實(shí)驗(yàn)結(jié)果僅供科研參考,不推薦用于臨床診斷結(jié)果。
?ELISA常用的方法:雙抗體夾心法、間接法、競(jìng)爭(zhēng)法以及BAS-ELISA等,子科生物ELISA產(chǎn)品主要用雙抗體夾心法。
深圳子科生物科技有限公司專業(yè)研發(fā)生產(chǎn)酶聯(lián)免疫小鼠血小板活化因子(PAF)ELISA試劑盒,ELISA 檢測(cè)的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù),為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性,在實(shí)驗(yàn)過(guò)程中必須堅(jiān)持全面質(zhì)量控制和全過(guò)程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。
子科生物技術(shù)部總結(jié)介紹如下:
注意事項(xiàng):
●每個(gè)標(biāo)本量收集體積= 100ul × 檢測(cè)種類,如要做復(fù)孔,標(biāo)本量收集體積= 100ul × 檢測(cè) 種類×2 。
● 對(duì)于作某一個(gè)具體指標(biāo)來(lái)說(shuō),先用一系列稀釋度作一批標(biāo)本測(cè)定,得出對(duì)實(shí)驗(yàn)有意義 的一個(gè)稀釋度(即測(cè)定劑量反應(yīng)曲線),然后用一個(gè)zui適稀釋度測(cè)定即可。
● 收集標(biāo)本前必須清楚要檢測(cè)的成份是否足夠穩(wěn)定。對(duì)收集后當(dāng)天進(jìn)行檢測(cè)的標(biāo)本,儲(chǔ)存在 4 ℃ 備用,如有特殊原因需要周期收集標(biāo)本,將標(biāo)本及時(shí)分裝后放在 -20 ℃ 或 -70 ℃ 條 件下保存。避免反復(fù)凍融。標(biāo)本 2 -8 ℃ 可保存 48 小時(shí), -20 ℃ 可保存 1 個(gè)月。 -70 度 可保存 6 個(gè)月。部分激素類標(biāo)本需添加抑肽酶。
● 血清標(biāo)本采集時(shí),應(yīng)注意避免溶血,紅細(xì)胞溶解時(shí)會(huì)釋放出具有過(guò)氧化物酶活性的物質(zhì), 以 HRP 為標(biāo)記的 ELISA 測(cè)定中,溶血標(biāo)本可能會(huì)增加非特異性顯色。
●為了保證尿液檢測(cè)結(jié)果的準(zhǔn)確性,必須正確收集尿液標(biāo)本和保存。盛尿容器要清潔干燥。 使用一次性的容器(如塑料尿杯),避免因用藥并清洗不干凈而造成的污染,影響檢 測(cè)結(jié)果。尿液標(biāo)本必須新鮮,留取后,應(yīng)及時(shí)檢測(cè)或保存,以免細(xì)菌繁殖。因在室溫中久 置后(尤其是夏季)尿中磷酸鹽等可析出結(jié)晶而干擾檢測(cè)。
● 標(biāo)本宜在新鮮時(shí)檢測(cè)。如有細(xì)菌污染,菌體中可能含有內(nèi)源性 HRP,也會(huì)產(chǎn)生假陽(yáng)性反應(yīng)。 保存過(guò)久可發(fā)生聚合在 ELISA 中可使本底加深。
● 凍結(jié)標(biāo)本融解后,蛋白質(zhì)局部濃縮,分布不均,應(yīng)充分混勻宜輕緩,避免氣泡,可上下顛 倒混和,不要在混勻器上強(qiáng)烈振蕩。
● 混濁或有沉淀的標(biāo)本應(yīng)先離心或過(guò)濾,澄清后再檢測(cè)。
● 反復(fù)凍融會(huì)使蛋白效價(jià)降低,所以待測(cè)標(biāo)本如需保存作多次檢測(cè),宜少量分裝冰存。也可 加入適當(dāng)防腐劑。
● 如果您在實(shí)驗(yàn)中遇到任何特殊問(wèn)題,敬請(qǐng)咨詢我公司深圳子科生物技術(shù)部。
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