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產(chǎn)品介紹 | |||
| 品名 | Rabbit anti-calretinin | ||
| 貨號(hào) | 7697 | ||
| 規(guī)格 | 200 µl、500 µl、1mL | ||
| 克隆性 | 多克隆 | ||
| 共軛 | 非共軛 | ||
| 反應(yīng)性 | 人、鼠、leopard danio | ||
| 應(yīng)用 | 免疫組織化學(xué)、免疫組織化學(xué)-石蠟切片、免疫組織化-冷凍切片 | ||
| 宿主動(dòng)物 | 兔 | ||
| 抗體形式 | 血清凍干 | ||
| 產(chǎn)品形式 | 凍干全血清（不含防腐劑） | ||
| 復(fù)溶 | 用200µl雙蒸餾水 | ||
| 產(chǎn)品描述 | Rabbit anti-calretinin通過(guò)用在N-末端含有6-his標(biāo)簽的重組人鈣視網(wǎng)膜蛋白免疫，在兔中產(chǎn)生針對(duì)鈣視網(wǎng)膜蛋白的抗血清。該抗體的特異性和效力進(jìn)行了評(píng)估：a）通過(guò)生Wu素-阿維丁標(biāo)記4%多聚甲醛固定腦的冷凍態(tài)、振動(dòng)體和鏈烷烴切片，以及b）通過(guò)免疫印跡的免疫酶標(biāo)記 | ||
| 稀釋 | 疫組織化學(xué)：在多聚甲醛（4%）或福爾馬林固定的組織、冷凍狀態(tài)或石蠟切片上進(jìn)行1:2’000-1:5’000。 免疫印跡：1:1’000-1:2’000。 對(duì)于免疫組織化學(xué)和免疫印跡，使用親和素-生Wu素法測(cè)定滴度。我們建議通過(guò)滴定實(shí)驗(yàn)來(lái)確定稀釋度。 | ||
| 儲(chǔ)存 | 重構(gòu)后，冷凍成小份（如1µl），并保持在-80°C（或至少-20°C）。使用時(shí)，保持在4°C（含0.01%疊Dan化鈉）。避免反復(fù)冷凍和解凍。 | ||
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