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產品介紹 | |||
| 品名 | Rabbit anti-calretinin | ||
| 貨號 | 7697 | ||
| 規格 | 200 µl、500 µl、1mL | ||
| 克隆性 | 多克隆 | ||
| 共軛 | 非共軛 | ||
| 反應性 | 人、鼠、leopard danio | ||
| 應用 | 免疫組織化學、免疫組織化學-石蠟切片、免疫組織化-冷凍切片 | ||
| 宿主動物 | 兔 | ||
| 抗體形式 | 血清凍干 | ||
| 產品形式 | 凍干全血清（不含防腐劑） | ||
| 復溶 | 用200µl雙蒸餾水 | ||
| 產品描述 | Rabbit anti-calretinin通過用在N-末端含有6-his標簽的重組人鈣視網膜蛋白免疫，在兔中產生針對鈣視網膜蛋白的抗血清。該抗體的特異性和效力進行了評估：a）通過生Wu素-阿維丁標記4%多聚甲醛固定腦的冷凍態、振動體和鏈烷烴切片，以及b）通過免疫印跡的免疫酶標記 | ||
| 稀釋 | 疫組織化學：在多聚甲醛（4%）或福爾馬林固定的組織、冷凍狀態或石蠟切片上進行1:2’000-1:5’000。 免疫印跡：1:1’000-1:2’000。 對于免疫組織化學和免疫印跡，使用親和素-生Wu素法測定滴度。我們建議通過滴定實驗來確定稀釋度。 | ||
| 儲存 | 重構后，冷凍成小份（如1µl），并保持在-80°C（或至少-20°C）。使用時，保持在4°C（含0.01%疊Dan化鈉）。避免反復冷凍和解凍。 | ||
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