產(chǎn)品介紹 | |||
品名 | Rabbit anti-Calbindin D-28k | ||
貨號 | CB-38a (new batch) | ||
規(guī)格 | 200 µl | ||
克隆性 | 多克隆 | ||
共軛 | 非共軛 | ||
反應(yīng)性 | 人、大鼠、小鼠、豬 | ||
應(yīng)用 | 蛋白質(zhì)印跡、免疫組織化學(xué)、免疫細(xì)胞化學(xué)、免疫組織化-石蠟切片、免疫組織學(xué)-冷凍切片、免疫組化-游離漂浮切片 | ||
宿主動物 | 兔 | ||
抗體形式 | 血清凍干 | ||
產(chǎn)品形式 | 凍干的抗血清(不含防腐劑) | ||
復(fù)溶 | 200µl雙蒸餾水 | ||
產(chǎn)品描述 | Rabbit anti-Calbindin D-28k該抗血清是針對重組大鼠鈣結(jié)合蛋白D-28k(CB)產(chǎn)生的。它與許多其他物種的鈣結(jié)合蛋白D-28k發(fā)生交叉反應(yīng),包括人類、猴子、大鼠、小鼠、雞和魚類。在免疫印跡中,它識別大約27-28kDa的單個條帶。在相關(guān)蛋白鈣視網(wǎng)膜蛋白濃度非常高(比CB高10倍)的樣品中,蛋白質(zhì)印跡中可能出現(xiàn)更大的條帶(29-30kDa)。因此,根據(jù)抗血清的稀釋度和/或免疫染色的敏感性,也可以揭示這種其他鈣結(jié)合蛋白。如有疑問,我們建議改用針對鈣結(jié)合蛋白D-28k的Swant單克隆抗體300,該抗體不識別鈣視網(wǎng)膜蛋白。 | ||
稀釋 | 免疫組織化學(xué):抗生Wu素-生Wu素法1:10'000-1:20'00 免疫印跡:1:100-1:1’000。 我們建議通過滴定實驗來確定稀釋度。 | ||
儲存 | 制作小份(例如2-5µl)。對于長期儲存,應(yīng)保持在-80°C(或至少-20°C)。連續(xù)使用時,保持在4°C(含0.01%疊Dan化鈉)。避免反復(fù)冷凍和解凍。 |
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