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上海經科化學科技有限公司
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The Caspase-3 Colorimetric Assay Kit (Cat. No. L00289) provides a simple and convenient means of assaying the activity of caspase. Caspase (Cysteine-requiring Aspartate Protease)
DescriptionThe Caspase-3 Colorimetric Assay Kit (Cat. No. L00289) provides a simple and convenient means of assaying the activity of caspase. Caspase (Cysteine-requiring Aspartate Protease) is a protease family, and it plays a very important role in apoptosis. This kit is based on spectrophotometric detection of the chromophore pnitroanilide (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA can be quantified using a spectrophotometer or a microtiter plate reader at 400 nm or 405 nm. By comparing the absorbance of pNA from an apoptotic sample to an uninduced control, the relative increase of caspase-3 activity can be determined. |
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