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產品型號Z02019

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產品簡介

At least 23 different variants of Interferon-alpha are known. The individual proteins have molecular masses between 19,000-26,000 Da and consist of proteins with lengths of 156-166

詳細介紹

Full Name

HSA-IFN-α 2b, Human Serum Albumin and Interferon -α 2b

Abbreviated Name-1

HSA-IFN-α 2b

Abbreviated Name-2

HSA-IFNa2b, HSA-IFN a2b

Description

At least 23 different variants of Interferon-alpha are known. The individual proteins have molecular masses between 19,000-26,000 Da and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxyl-terminal end. GenScript Human Serum Albumin and Interferon （HSA-IFN）-α 2b produced in P. Pichia is a single non-glicosylated, polypeptide chain having a molecular mass of 85,700 Da.

Source

P. Pichia

M.W.

85,700 Da

Purity

Greater than 98.0% as determined by:
（a） Analysis by RP-HPLC
（b） Anion-exchange FPLC
（c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel

Endotoxin Level

Less than 0.1 ng/μg （IEU/μg） of HSA-IFN-α 2b

Specific Activity

HSA-IFN-α 2b is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 5×10⁷ IU/mg.

Storage

Lyophilized HSA-IFN-a2b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HSA-IFN-α 2b should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to ass a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles.

Formulation

Lyophilized from a （1 mg/ml） solution in containing 5.55 mg sodium phosphate dibasic, 5.55 mg sodium phosphate monobasic buffer, 296 mg sucrose and 0.37 mg Tween 80.

Reconstitution

It is recommended to reconstitute the lyophilized HSA-IFN a2b in sterile 18 MΩ-cm H₂O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions.