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上海經科化學科技有限公司
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Caog Peptide:eIF2α (Ab-51) pAb Blocking Peptide(RP19950)The synthesized non-phosphopeptide was derived from human eIF2α around the phosphorylation site of serine 51 (E-L-SP-R-R).
Description | Caog Peptide:eIF2α (Ab-51) pAb Blocking Peptide(RP19950)The synthesized non-phosphopeptide was derived from human eIF2α around the phosphorylation site of serine 51 (E-L-SP-R-R). | |
Concentration | 100ug/10 mM phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA, 5%DMF and 5% glycerol. | |
Quality Control | The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. | |
Applications | For Western blotting: add 10 ?l of antibody and 10 ?l of blocking peptide to 10 ml of antibody dilution buffer, and incubate at 4℃ over night or at room temperature for 2 hours before allowing to react with the blot. For immunohistochemistry: add 2 ?l of antibody and 2 ?l of blocking peptide to 0.096 ml of buffer, and incubate at 4℃ over night or at room temperature for 2 hours before allowing to react with the slides. | |
Specificity | The peptide specifically blocks the signal of Rabbit Anti eIF2α (Ab-51) compley in Western blotting and immunohistochemistry. | |
storage | Store at -20℃ |
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