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QuickClean 5M PCR Purification Kit

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產品簡介

The QuickClean 5M PCR Purification Kit is a reagent kit from GenScript. It contains all the necessary reagents, mini-columns, and collection tubes for the fast and reliable extraction

詳細介紹

Description:

The QuickClean 5M PCR Purification Kit is a reagent kit from GenScript. It contains all the necessary reagents, mini-columns, and collection tubes for the fast and reliable extraction of DNA from PCR reactions and for DNA cleanup in enzymatic reactions. In only five minutes, QuickClean can recover up to 20 μg of single- or double-stranded DNA product. DNA molecules ranging in size from 70 to 10,000 base pairs are adsorbed onto the QuickClean Column while impurities such as enzymes and proteins, small DNA fragments, dyes, salts, nucleotides and short oligonucleotides （such as primers and probes） are washed away.

Eluted in a small volume of low-salt buffer with complete removal of contaminants and inhibitors, the purified DNA is immediay ready for downstream applications such as PCR, transformation, restriction enzyme digestion, cloning, sequencing, in vitro translation and transfection.

Key Features:

Easy to perform: QuickClean's simple and rapid procedure purifies DNA in five minutes.
High capacity: Each column has a capacity of 20 μg DNA.
High purity: The kit compley removes contaminants and inhibitors.
Reproducible yields: Recovery is typically between 90% and 98%, reproducible every time.

Applications:

The QuickClean 5M PCR Purification Kit enables the fast purification of high-quality DNA products from PCR reactions and enzymatic reactions. The extracted DNA is immediay ready for further downstream applications such as the following:

PCR and cloning
Restriction enzyme digestion
Transformation
Sequencing
In vitro translation

Examples:

The is here compared to a commercially available kit （Competitor A） for rapid PCR DNA product purification from PCR reactions. Both kits were used following the protocols provided by the manufacturers. The results are shown in Figure 1.

Figure 1. The QuickClean 5M Purification Kit and a kit from competitor A were used for PCR DNA purification from PCR reactions. Both kits were used in accordance with the protocols provided by the manufacturers. Two PCR DNA purifications were performed using each kit. The same volume of PCR material was used in each purification. Lane 1 shows unpurified material.