污水處理設備 污泥處理設備 水處理過濾器 軟化水設備/除鹽設備 純凈水設備 消毒設備|加藥設備 供水/儲水/集水/排水/輔助 水處理膜 過濾器濾芯 水處理濾料 水處理劑 水處理填料 其它水處理設備
上海經科化學科技有限公司
上海經科化學科技有限公司
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pRNAT-U6.2/Lenti siRNA expression vector is compatible with the Invitrogen ViraPowerTM Lentiviral_Expression System. It is designed for siRNA expression in both dividing and nondividing cells.
| Description: pRNAT-U6.2/Lenti siRNA expression vector is compatible with the Invitrogen ViraPowerTM Lentiviral_Expression System. It is designed for siRNA expression in both dividing and nondividing cells. The siRNA insert can be conveniently cloned between the BamH I and Xho I sites under the control of the U6 promoter. The vector contains a neomycin resistance gene for establishing stable cell lines and a coral GFP gene for tracking transfection efficiency. Both of these are driven by CM and SV40 promoters, respectively. The vector uses HIV 3'LTR for viral transcription and a Rous sarcoma enhancer/promoter joined to HIV 5'LTR （PRSV/5'LTR） for packaging. |
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| Download Protocol:tm0175 |
| Forward Sequencing Primer: DA0011:pRNA-U6 Forward （TACGATACAAGGCTGTTAGAGAG） |
| Reverse Sequencing Primer: DA0012:pRNA Reverse （TAGAAGGCACAGTCGAGG） |
| Detailed Map: |
| 5' LTR:1-410 |
| 3' LTR:4906-5140 |
| ColE1 ori:7220-8106 |
| Polylinker:2200-2275 |
| U6 Promoter:1873-2199 |
| CMV Promoter:2320-2907 |
| cGFP:2924-3643 |
| SV40:3651-3996 |
| Neomycin:4037-4831 |
| Ampicillin:6300-7160 |
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