產品介紹:
SARTORIUS 瓶頂過濾器18083-E的詳細介紹
Sartolab RF/BT
Disposable PES Vacuum Filtration Units
Sartolab vacuum filtration units
In today’s tissue culture laboratory,
speed, convenience, sterility
assurance and cost effectiveness
are the key requirements for busy
end users and buyers.
Vivascience Sartolab Receiver Flask
(RF) and Bottle Top (BT) disposable
vacuum filter systems are the product
of choice for filtration of tissue culture
media, serum, buffers, antibiotics,
vitamin solutions and
other aqueous biologicals.
Sartolab RF and BT filter units offer
high quality Sartorius PES membranes,
combining highest flow-rates and
throughput with extremely low protein
binding and extractables. Not only
is this high performance membrane
unique to Vivascience Sartolab
products, it is also offered standardly
as a 90 mm membrane in all of our
500 ml and 1000 ml bottle top and
receiver flask units; guaranteeing
faster, higher throughput filtration
more cost effectively.
Flexible
Sterile filtration of cell culture media,
buffers, additives and other aqueous
biologicals.
PES Membrane of
choice for cell culture
PES membranes combine extremely
high flow rates and throughput with
low non-specific binding.
Ready-to-use and easy handling
Pre-sterilized filter units, require only
connection to a vacuum source.
User-friendly design eliminates
mistakes in usage.
Single-unit packaging
Receiver flasks are removable and
can be closed for storage with the
sterile caps supplied with the units.
Bottle tops will fit any 45 mm diameter
sterile storage bottle.
Free of cytotoxic effects
Sartolab units are free of cytotoxic
effects, they pass all USP and
cytotoxicity tests.
Highest flow rates
The PES membrane in all units and
a 90 mm filter diameter in 500 ml
and 1000 ml units increase the speed
and throughput of filtration.
Sartolab Receiver Flask (RF) Range
Sartolab RF1000
1000 ml capacity
Cat No. 18083-E
Sartolab RF150
150 ml capacity
Cat No. 18081-E
Sartolab RF500
500 ml capacity
Cat No. 18082-E
0
50
100
150
200
CN Nylon SFCA Other PES
Provider
PES Vivascience
μg/cm2
0
200
400
600
800
1000
1200
0 20 40 60 80 100
Time (seconds)
Volume (ml)
Competitor M (DMEM)
Competitor M (RPMI + 10% FCS)
Vivascience (RPMI + 10% FCS)
Vivascience (DMEM)
IgG (μg/cm2) BSA (μg/cm2)
• 150, 500, 1000 ml
• 0.2 μm pore size
• Polystyrene housing
• PES (Polyethersulphone) membrane
• 50 mm & 90 mm diameter membrane
• 45 mm bottle-top fitting (Sartolab BT)
• Gamma sterilized
• Pack of 12
Sartolab Bottle Top (BT) Range
PES Membrane - Low Protein Binding Highest Flow Rates
Sartolab BT1000
1000 ml capacity
Cat No. 18086-E
Sartolab BT150
150 ml capacity
Cat No. 18084-E
Sartolab BT500
500 ml capacity
Cat No. 18085-E
103 Ø
240
122
120 Ø
163
322 2
85 Ø
157
91
Dimensions (mm)
Specifications
Capacity 150 ml, 500 ml, 1000 ml funnel and flask
Membrane PES (Polyethersulphone)
50 mm and 90 mm diameter
0.2 μm pore size
Housing Polystyrene
Connection to vacuum Plug-on hose nipple
Cytotoxicity All materials pass USP class
VI and cytotoxicity ISO 10993 tests
Neck size bottle top units 45 mm diameter
Sterilization Gamma radiation
Filter Unit with PES Membrane, Pore size 0.2 μm
Cat. No. Volume capacity PES Membrane diameter Pack size
18081-E 150 ml 50 mm 12
18082-E 500 ml 90 mm 12
18083-E 1000 ml 90 mm 12
Bottle Top Filter with PES Membrane, Pore size 0.2 μm
Cat. No. Volume capacity PES Membrane diameter Pack size
18084-E 150 ml 50 mm 12
18085-E 500 ml 90 mm 12
18086-E 1000 ml 90 mm 12
Publication No.: SLU1503-e02042 · Order No.: 85030-515-53 · sart-099
sartorius Sartocon Slice 200 實驗室切向流過濾系統(tǒng)的詳細介紹
實驗室切向流過濾系統(tǒng)
處理量:30-5000ml
夾具特點
· Sartocon Slice200所有與料液接觸的部位均由生物安全性材料不銹鋼316L構造。
·選用Luer lock接口,保證了系統(tǒng)在壓力條件下操作的安全性。
·低殘留體積設計(<2ml),減小工作體積,提高產品收濾。
·可調節(jié)的支腳設計,放置穩(wěn)定。
·可以放置2塊Sartocon Slice200膜包,過濾面積0.04m2。
·與生產用Sartocon 選用同樣的膜材料和同樣的流道設計,確保放大生產的連續(xù)性,可靠性和安全性。
·加大的橢圓型進口設計,有效避免air-lock,保證系統(tǒng)*清洗。
·膜包采用彈性的硅膠密封,因此膜包之間無需額外加入硅膠墊片,操作簡單。
·豎直夾置膜包,可以將系統(tǒng)中殘余液體*排空。
膜包特點
·膜包有效過濾面積 0.02m2
·膜包密封采用硅橡膠的密封技術,不使用膠粘劑,膜包的生物安全性好。
·膜包的熱穩(wěn)定性好,是市場上*能夠進行多次在線蒸汽滅菌的膜包。
·可以耐受長時間1N NaOH的清洗和除熱源處理。
·膜包的構造上,采用增大湍流的結構,有效地抑止了凝膠層形成,從而提高膜包的過濾速度。
·膜包的進液口形狀為橢圓型,與傳統(tǒng)的圓形流口相比,流口面積增大,壓降減小,流速得到進一步提高。
·膜包間密封不需使用硅膠墊片,避免產生衛(wèi)生死角,同時操作更為簡便。
· Sartocon 系列膜包還可適用于其它公司的切向流系統(tǒng),但是由于設計上的不同,對膜包施加的力矩也有相應改變。
膜包材質:
·
聚醚砜(PESU)膜包——采用性能優(yōu)異的親水PESU超濾膜,蛋白質非特異吸附性低,蛋白質損失小,產品回收濾高。化學兼容性好,可以在PH1-14的全范圍內使用。
PESU膜包的規(guī)格齊全,有截留分子量1K/5K/8K/10K/30K/50K/100K/300K/0.1μm 膜可供選擇。
·
Hydrosart膜包——采用SartoriusHydrosart膜,Hydrosart既保持了傳統(tǒng)的纖維素材料蛋白非特異性吸附低的優(yōu)點,又克服了纖維素材料化學兼容性差的缺點,可在PH2-14
酸堿度范圍內使用。
超濾膜包截留分子量5K/10K/30K,微笑濾膜包0.2μm/0.45μm。可選Open-channel微濾膜包。
產品簡介:
切向流超濾(Cross Flow Ultra-Filtration)
切向流過濾是指液體流動方向與過濾方向呈垂直方向的過濾形式。傳統(tǒng)的液體死端過濾(dead
end)是大部分微孔過濾(MF,微濾),包括除菌過濾所采用的過濾形式,其液體的流動方向與過濾方向*,隨著過濾的進行,過濾膜表面形成的濾餅層或凝膠層厚度逐漸增大,流速逐漸降低。當過濾介質為孔徑細小的超濾膜或微濾膜時料液中固形物含量很高時,采取死端過濾方式,流速將急速降低,因此死端過濾只能處理小體積的料液。對于較大規(guī)模的料液過濾時,就需要采用切向流過濾方式,液體流動在過濾介質表面產生剪切力,減小了濾餅層或凝膠層的堆積,保證了穩(wěn)定的過濾速度。因此且切向流過濾方式被廣泛地應用于超濾(UF)和部分的微濾(MF)的處理過程。
切向流過濾
死端過濾
切向流超濾的應用領域
切向流超濾系統(tǒng)主要應用于生物制品的濃縮、提純、透析(脫鹽和脫醇等)、置換緩沖液、培養(yǎng)液和緩沖液除熱源等
切向流微濾系統(tǒng)主要應用于生物工程領域下游處理,如取代離心機從發(fā)酵液中收集細胞及去除細胞碎片等,特別是賽多利斯的切向流MF系統(tǒng)因其能夠整體在位蒸汽滅菌,還可以應用于動物細胞連續(xù)培養(yǎng)的無菌換液以及提高菌體分泌物表達收率,在培養(yǎng)過程中無菌條件下的連續(xù)換液。
賽多利斯的切向流超濾膜和超濾系統(tǒng)
賽多利斯的切向流超濾膜
膜類型Hydrosart膜聚醚砜膜三醋酸纖維素膜
特點膜材料中zui低的蛋白吸附
廣泛的pH范圍,2~14
高流速
使用壽命長
易再生清洗
可蒸汽消毒滅菌較低的蛋白吸附
適用所有pH范圍
高流速
可蒸汽消毒滅菌
易再生清洗
使用壽命長極低的蛋白吸附
高流速
易再生清洗
截留分子量1KD/5KD/8KD/10KD/20KD/30KD/50KD/100KD/300KD
孔徑0.2μm和0.45μm除菌------
過濾面積Sartocon: 0.6m2,Sartocon OC: 0.4m2;
Sartocon Slice: 0.1m2,Slice拋棄型:0.1m2Sartocon: 0.7m2,
Sartocon Slice: 0.1m2,Slice拋棄型:0.1m2Sartocon: 0.7m2,
Sartocon Slice: 0.1m2
賽多利斯的切向流超濾系統(tǒng)
超濾系統(tǒng)Sartocon-Slice拋棄型Sartocon-Sclice系統(tǒng)Sartocon系統(tǒng)
適用范圍實驗室小試實驗室和小規(guī)模生產規(guī)模化生產
處理量0.5~100L1~100L5~1,000L,2,000L