大鼠胃泌素(Gastrin)ELISA試劑盒
Rat Gastrin ELISA Kit
實驗目的：本試劑盒僅供研究使用，用于測定大鼠血清，血漿及相關液體樣本中胃泌素(Gastrin)的含量。
實驗原理：本試劑盒應用雙抗體夾心法測定標本中大鼠胃泌素(Gastrin)水平。用純化的大鼠胃泌素(Gastrin)抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入胃泌素(Gastrin)，再與HRP標記的胃泌素(Gastrin)抗體結合，形成抗體-抗原-酶標抗體復合物，經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉化成藍色，并在酸的作用下轉化成zui終的黃色。顏色的深淺和樣品中的胃泌素(Gastrin)呈正相關。用酶標儀在450nm波長下測定吸光度（OD值），通過標準曲線計算樣品中大鼠胃泌素(Gastrin)濃度。
Purpose
This kit allows for the determination of Gastrin concentrations in Rat serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Rat Gastrin level in the sample，use Purified Rat Gastrin antibody to coat microtiter plate wells, make solid-phase antibody, then add Gastrin to wells, Combined Gastrin which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Gastrin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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