大鼠中性粒細(xì)胞彈性蛋白酶（NE）酶聯(lián)免疫分析(ELISA)試劑盒
實(shí)驗(yàn)目的：本試劑盒僅供研究使用，用于測(cè)定大鼠血清，血漿及相關(guān)液體樣本中中性粒細(xì)胞彈性蛋白酶（NE）含量。
實(shí)驗(yàn)原理：本試劑盒應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中大鼠中性粒細(xì)胞彈性蛋白酶（NE）水平。用純化的大鼠中性粒細(xì)胞彈性蛋白酶（NE）抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入中性粒細(xì)胞彈性蛋白酶（NE），再與HRP標(biāo)記的中性粒細(xì)胞彈性蛋白酶（NE）抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的中性粒細(xì)胞彈性蛋白酶（NE）呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（OD值），通過標(biāo)準(zhǔn)曲線計(jì)算樣品中大鼠中性粒細(xì)胞彈性蛋白酶（NE）濃度。
試劑盒性能：
1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.92以上。
2.批內(nèi)與批見應(yīng)分別小于9%和15%
Drug Names
Generic Name：Rat neutrophil elastase (NE) ELISA Kit.
Purpose
This kit allows for the determination of NE concentrations in Rat serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Rat NE level in the sample，use Purified Rat NE antibody to coat microtiter plate wells, make solid-phase antibody, then add NE to wells, Combined NE which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NE in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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