牛磷酸果糖激酶(PFK)ELISA試劑盒實(shí)驗(yàn)原理：本試劑盒用于測定牛血清，細(xì)胞上清及相關(guān)液體樣本中磷酸果糖激酶(PFK)的活性。應(yīng)用雙抗體夾心法測定標(biāo)本中牛磷酸果糖激酶(PFK)水平。用純化的牛磷酸果糖激酶(PFK)抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入磷酸果糖激酶(PFK)，再與HRP標(biāo)記的磷酸果糖激酶(PFK)抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的磷酸果糖激酶(PFK)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（OD值），通過標(biāo)準(zhǔn)曲線計(jì)算樣品中牛磷酸果糖激酶(PFK)濃度。
Bovine Phosphofructokinase (PFK) ELISA Kit
Principle of the assay:
This kit allows for the determination of PFK concentrations in Bovine serum, cell culture supernatant, and other biological fluids.Assay Bovine PFK level in the sample，use Purified Bovine PFK antibody to coat microtiter plate wells, make solid-phase antibody, then add PFK to wells, Combined PFK antibody which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PFK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
試劑盒供應(yīng)商：上海華壹生物科技有限公司
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