小鼠雙氫睪酮(DHT)ELISA試劑盒
實(shí)驗(yàn)原理：
本試劑盒用于測(cè)定小鼠血清，血漿及相關(guān)液體樣本中雙氫睪酮(DHT)的含量。應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中小鼠雙氫睪酮(DHT)水平。用純化的小鼠雙氫睪酮(DHT)抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入雙氫睪酮(DHT)，再與HRP標(biāo)記的雙氫睪酮(DHT)抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的雙氫睪酮(DHT)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（OD值），通過標(biāo)準(zhǔn)曲線計(jì)算樣品中小鼠雙氫睪酮(DHT)濃度。
Mouse Dihydrotestosterone(DHT)ELISA Kit
Purpose:
This kit allows for the determination of DHT concentrations in Mouse serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Mouse DHT level in the sample，use Purified Mouse DHT to coat microtiter plate wells, make solid-phase antibody, then add DHT to wells, Combined DHT antibody which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DHT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
試劑盒供應(yīng)商：上海華壹生物科技有限公司
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