Rabbit Anti-phospho-JNK1/2/3 （pThr183/pTyr185）
Catalog Number: Rs-1640R
Quantity size: 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）
Background:
JNK1 （MAPK8） is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha （TNF-alpha） is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrome c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. JNK1 is activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. The JNK pathway is critically involved in diabetes and levels are abnormally elevated in obesity. The cell-permeable JNK inhibitory peptide may have promise as a therapeutic agent for diabetes.
Specificity :
·anti-phospho-JNK1/2/3 （pThr183/pTyr185） is a rabbit monoclonal antibody unconjugated
· specific for phospho-JNK1/2/3 （pThr183/pTyr185） of human, mouse, rat, goat, dog
· epitope mapping is synthetic phospho-peptide corresponding to residues surrounding （pThr183/pTyr185） of human, mouse, rat, goat, dog JNK1/2/3
· use for western blotting, elisa, immunoprecipitation and immunohistochemistry
· Protein G affinity chromatography purification, purity :>95%
· Isotype: IgG
· mol wt: 36kDa
Application :
· Western blotting 1:100-500
· Immunohistochemistry 1:100-500
· ELISA 1:500-1000
· Optimal working dilutions must be determined by the end user.
Storage:
Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
