日韩午夜在线观看,色偷偷伊人,免费一级毛片不卡不收费,日韩午夜在线视频不卡片

上海瑞齊生物科技有限公司

Human Procalcitonin (PCT)

時間:2011-9-5閱讀:1142
分享:

 

RD
Human Procalcitonin (PCT)

 
FOR RESEARCH USE ONLY
 
Assay range40 ng/L - 1200 ng/L                96determinations
Purpose
This kit allows for the determination of PCT concentrations in Humanserum, cellculture supernates and other biological fluids
 
Human Procalcitonin (PCT) Principle of the assay
The kit assay MousePCT level in the sample,use Purified PCT antibody to coat microtiter plate wells, make solid-phase antibody, then addPCTto wells,CombinedPCT antibody which With HRP labeled,become antibody –antigen - enzyme- antibody complex, after washing Compley,Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed,reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration ofPCTin the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Procalcitonin (PCT) Materials provided with the kit

1
wash solution
20ml×1bottle
7
Stopp Solution
6ml×1 bottle
2
HRP-Conjugate reagent
6ml×1 bottle
8
Standard2400 ng/L
0.5ml×1 bottle
3
Microelisa stripplate
12well×8strips
9
Standard diluent
1.5ml×1bottle
4
Sample diluent
6ml×1 bottle
10
Instruction
1
5
Chromogen Solution A
6ml×1 bottle
11
Closure plate membrane
2
6
Chromogen Solution B
6ml×1 bottle
12
Sealed bags
1

Specimen requirements
1.       extractas soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
2.       Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1.       Dilute and add sample:Dilute Original density Standard as follow table:

1200 ng/L
5 Standard
150μl Original density Standard+150μl Standard diluent
600 ng/L
4 Standard
150μl 5 Standard+150μl Standard diluent
300 ng/L
3 Standard
150μl 4 Standard+150μl Standard diluent
150 ng/L
2 Standard
150μl 3 Standard +150μl Standard diluent
75 ng/L
1 Standard
150μl 2 Standard +150μl Standard diluent

2.add sampleSet blank wells separay (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37.
4.Configurate liquid: 30-fold wash solution diluted 30-foldwith distilled water and reserve.
5.washingUncover Closure plate membrane, discardLiquid, dry by swing, add washing buffer to every well, still for 30s then drain,repeat 5 times, dry by pat.
6.add enzymeAdd HRP-Conjugate reagent 50μl to each well, except  blank well.
7.incubateOperation with 3.
8.washingOperation with 5.
9.colorAdd Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37
10.Stop the reactionAdd Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assaytake blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
Steps description

Standard, Sample diluent

 

AddStandard, Sample diluent, incubate for 30 min at 37.

 

Wash 5 time,AddHRP-Conjugate reagent, incubate for 30 min at 37.

 

Wash 5 times,Add Chromogen Solution A and B, incubate for 30 min at 37.

 

AddStopp Solution

 

Read absorbance at 450nm within 15 min

 

calculate

Calculate
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
Important notes
1.       The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2.       washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3.       add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use Volley .
4.       if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluenteand multiplied by the dilution factor.×n×5.
5.       Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6.       The substrate evade the light preservation.
7.       Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8.       All samples, washing buffer and each kind of reject should according to infective material process.
9.       Do not mix reagents with those from other lots.
 
Storage and validity
1Storage 2-8℃.
2validity six months
 

會員登錄

×

請輸入賬號

請輸入密碼

=

請輸驗證碼

收藏該商鋪

X
該信息已收藏!
標簽:
保存成功

(空格分隔,最多3個,單個標簽最多10個字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時間回復您~

以上信息由企業自行提供,信息內容的真實性、準確性和合法性由相關企業負責,環保在線對此不承擔任何保證責任。

溫馨提示:為規避購買風險,建議您在購買產品前務必確認供應商資質及產品質量。

在線留言
主站蜘蛛池模板: 雷州市| 钟山县| 南漳县| 武夷山市| 磴口县| 徐闻县| 拜泉县| 淅川县| 洪泽县| 宜川县| 永新县| 铜陵市| 馆陶县| 龙山县| 蒲江县| 二手房| 祁连县| 龙州县| 凯里市| 武安市| 扎兰屯市| 东阳市| 平南县| 肃南| 清新县| 喀喇沁旗| 黄梅县| 桐柏县| 山西省| 宜章县| 闻喜县| 扶余县| 巴林左旗| 永宁县| 讷河市| 永城市| 永州市| 普格县| 平潭县| 怀仁县| 科技|