Mouse Anti-NSE
（Neuron Specific Enolase）
BD
Cat. Number: Rs-1027R
Quantity size: 0.2ml （dilute with pH 7.4 0.01 M PBS or diluent of antibody）
Background: This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. SUBUNIT: Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific. Subcellular location: Cytoplasm （By similarity）. Cell membrane （By similarity）. Note=Can translocate to the plasma membrane in either the homodimeric （alpha/alpha） or heterodimeric （alpha/gamma） form （By similarity）. TISSUE SPECIFICITY: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.developmental stage: During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. Belongs to the enolase family.
Specificity: · anti- NSE is a mouse monoclonal antibody unconjugated
· specific for NSE of human, rat and chicken
· epitope mapping near the C terminus of NSE of human, rat
and chicken
· human, rat, and chicken cross reacted
· use for western blotting, elisa, immunoprecipitation
and immunohistochemistry
· Protein A affinity chromatography purification, purity :>95%
· Isotype: IgG · mol wt: 48kDa
Application: · Western blotting: 1:100-500
· Immunohistochemistry: 1:100-500
· ELISA: 1:500-1000
· Optimal working dilutions must be determined by the end user.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Mouse Anti-NSE Neuron Specific Enolase）
