Mouse Anti- IFN-α2b抗體說明書

FROM: ABCAM

Cat. Number:  RS-0536R

Quantity size:  0.1ml  （dilute with pH 7.4    0.01 M PBS or diluent of antibody）

Background:  Interferon alpha 2b is one of the known 23 different variants of IFN-alpha. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.

Interferon alpha 2b is a potent immune modulator. It can either inhibit or enhance immune cell activity within the tightly regulated microenvironment of inflammation, depending upon the concentration of the cytokine and the activation stage of the cell. Interferon alpha 2B is currently used as an anticancer drug due to it's antiproliferative activity.

Specificity:      ·anti- IFN-α2b is a mouse monoclonal antibody unconjugated

                ·specific for IFN-α2b of  human  

                ·epitope mapping near the amino terminus of IFN-α2b  of  

                   human

                · use for western blotting, elisa, immunoprecipitation    

                   and immunohistochemistry

·  Protein A affinity chromatography purification, purity :>95%

· Isotype: IgG

· mol wt: 82kDa

Application:     · Western blotting: 1:100-500

· Immunohistochemistry: 1:100-500

· ELISA: 1:500-1000

·  Optimal working dilutions must be determined by the end user.

Storage:   Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.

Important Note:  This product as supplied is  intended for research use only, not for use in human, therapeutic or diagnostic applications.

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