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F0004-1瓊脂糖3：1HRB/Agarose 3：1 HRB
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產(chǎn)品介紹
Description
Analysis Note The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis （EEO） - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Application Ideal for the separation of small DNA fragments （e.g., PCR products） differing in size by as little as 2% and compares to the resolution of DNA in polyacrylamide gels. High resolution agarose is also ideal for resolving amplification fragment length polymorphisms （AmpFLP）, short tandem repeats （STR） and tri- and tetranucleotide repeats.
Low ethidium bromide and SYBR Green background staining
Properties
foreign activity DNase, RNase, NICKase, none detected
Safety
Personal Protective Equipment Eyeshields, Gloves, type N95 （US）, type P1 （EN143） respirator filter
WGK Germany 3
References
reference RegBook 1 （1）, 203:C
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