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Description
Analysis Note Binding capability: Streptavidin binds one molecule of biotin per subunit. Comparison of biotin binding and absorbance measurements indicates that ≤5% binding sites are occupied.
Other Notes Review: Application of the avidin-biotin technique in microbiology1; Protein isolated from the bacterium Streptomyces avidinii, having a high affinity for biotin2; Avidin-Biotin technology 3
Packaging The sales quantitites 1 mg and 5 mg correspond to ′mg protein′
Unit Definition 1 U corresponds to the amount of protein which binds 1μg (+)-biotin at pH 7.5
Properties
quality affinity purified
lyophilized from 10 mM potassium phosphate
mol wt Mr ~60000
solubility H2O: 0.1 mg/mL, clear, colorless
storage temp. −20°C
Safety
Personal Protective Equipment Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK Germany 3