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Description
Analysis Note For assay method, see McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969).
Biochem/physiol Actions Catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. Plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.2,3
Unit Definition One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min.
Physical form Lyophilized powder containing potassium phosphate buffer salts
Properties
form lyophilized powder
composition Protein, ~98% biuret
pI 4.95
storage temp. −20°C
Safety
Personal Protective Equipment Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Safety Statements 22-24/25
WGK Germany 3