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重組大鼠白介素-12/IL 12 Rat

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重組大鼠白介素-12/IL 12 Rat產品編號：CYT-390
產品規格：2μg/10μg/0.1mg

詳細介紹

重組大鼠白介素-12/IL 12 Rat

產品編號：CYT-390
產品規格：2μg/10μg/0.1mg

Catalogue Number	CYT-390
Synonyms	NKSF, CTL maturation factor （TCMF）, Cytotoxic lymphocyte maturation factor （CLMF）, TSF, Edodekin-alpha, IL-12.
Introduction	IL-12 is a heterodimeric cytokine that stimulates the production of interferon gamma from T-cells and natural killer cells, and also induces differentiation of Th1 helper cells. It is an initiator of cell-mediated immunity.
Description	Interleukin-12 Rat Recombinant produced in Sf9 insect cells is a glycosylated disulfide linked heterodimeric polypeptide containing 503 amino acids and having a molecular weight of 70 kDa comprised of disulfide-bonded 35 kDa （p35） and 40 kDa （p40） subunits. The IL-12 is purified by proprietary chromatographic techniques.
Source	Baculovirus.
Physical Appearance	Sterile Filtered White lyophilized （freeze-dried） powder.
Formulation	The protein （1mg/ml） was lyophilized with no additives.
Solubility	It is recommended to reconstitute the lyophilized Interleukin 12 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability	Lyophilized Interleukin-12 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL12 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.
Purity	Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence	The sequence of the first five N-terminal amino acids was determined and found to be Arg-Val-Ile-Pro-Val at the p35 subunit and Met-Thr-Glu-Leu-Glu at the p40 subunit.
Biological Activity	ED50 range = 0.01-0.05 ng/ml corresponding to a specific activity of 20,000,000-100,000,000IU/mg as determined by the dose dependent secretion of interferon-gamma from PHA and IL-2 activated mouse splenocytes that have been costimulated with PMA. Optimal concentration for individual application should be determined by a dose response assay.
Usage	ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.