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IL-10 Porcine 重組豬白介素-10/IL 10 Porcine

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重組豬白介素-10/IL 10 Porcine產(chǎn)品編號：CYT-404
產(chǎn)品規(guī)格：2μg/10μg/0.1mg

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重組豬白介素-10/IL 10 Porcine

產(chǎn)品編號：CYT-404
產(chǎn)品規(guī)格：2μg/10μg/0.1mg

Catalogue Number	CYT-404
Synonyms	B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.
Introduction	IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
Description	Interleukin-10 Porcine Recombinant produced in E.coli is a single, glycosylated polypeptide chain containing 160 amino acids and having a molecular mass of 18458 Dalton. The IL-10 is purified by proprietary chromatographic techniques.
Source	Escherichia Coli.
Physical Appearance	Sterile Filtered White lyophilized （freeze-dried） powder.
Formulation	The protein was lyophilized with no additives.
Solubility	It is recommended to reconstitute the lyophilized Interleukin 10 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability	Lyophilized Interleukin-10 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL10 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.
Purity	Greater than 95.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE.
Amino acid sequence	The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Ile-Lys-Ser.
Biological Activity	The ED50 as determined by the dose-dependant stimulation of MC/9 cell which was found to be < 15ng/ml corresponding to a Specific Activity of 66,667IU/mg.
Protein content	Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.36 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a calibrated solution of IL-10 as a Reference Standard.
Usage	ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.