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己糖激酶/Hexokinase
CAS Number : 9001-51-8
Size：500U
Units/mg Protein：426U/mg
Storage Temperature: -20°C
Synonym: ATP:D-hexose 6-phosphotransferase
Product Description:
Glycolysis is the process occurring in almost all living organisms by which they metabolize
D-glucose to generate energy and metabolic intermediates. In the first step of glycolysis, hexokinase
phosphorylates the C6 position of D-glucose in the presence of ATP by the following reaction: ATP +
D-glucose→ADP + D-glucose6-phosphate. Yeast hexokinase has three isozymes, designated P-I, P-II, and glucokinase (Glk1). Each has
distinctive properties. Yeast hexokinase P-II has both a catalytic and a regulatory function. Several other hexoses can serve as substrates for hexokinase (relative reaction rates):
Glucose;2-deoxy-2-fluoro-D-glucose; mannosamine; 5-thioglucose; 3-deoxy-3-aminoglucose;
1,5-anhydro-D-glucitol, 1-thio-D-glucose. Molecular Weight:110 kDa (dimer) Hexokinase is a dimeric protein with two equal 55 kDa

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monomers. Activators:Mg
2+
(KM = 2.6 mM), catecholamine-related compounds. Inhibitors:D-glucosamine ;D-mannose;D-xylose; 6-deoxy-D-glucose ;N-acetylmannoseamine. Also sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, lyxose, and thiol reactive compounds. Hexokinase is used for the determination of D-glucose, D-fructose, and D-sorbitol infood or other
biological materials. Hexokinase can also be used in the assay of glycosides that are convertible to glucose
or fructose. Unit Definition: One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C. Preparation Instructions:
Hexokinase is soluble in cold water (0.5-1.0 unit/ml) or citrate buffer, pH 7.0. Storage/Stability:
Store the product at –20 °C and it is stable for at least 2 years when stored properly. Solutions in water or citrate buffer have remained stable during repeated freezing and thawing for a
period of 30 days. Precautions and Disclaimer:
For Laboratory Use Only. Not for drug, household or other uses.

己糖激酶現貨供應，己糖激酶