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江蘇寶萊生物科技有限公司
胺氧化酶是一種活性蛋白。其主要作用是將氨基酸等形成的各種胺進一步氧化還原。Z適pH7.0(乙胺)、低于或等于60℃時該酶穩定,KM(乙胺)為0.088毫摩/升。受羰基試劑(氨基脲、羥胺、肼)等的抑制。對多胺、二級胺和四級胺沒有活性。經純化的酶為二聚體,相對分子質量均為8l 000。町緩慢氧化酪胺、組胺和其他二胺,在茶葉中與氮化合物代謝有關。
[產品名稱] | 胺氧化酶 |
[規格] | BR,17Tabor u/mg |
[包裝] | 600U |
英文名稱:PAO;Plasma Amine Oxidase
級別:BR
來源:Bovine Plasma
Specific activity:≥17Tabor units/mg
活力定義:1 international unit oxidizes 1?mole of benzylamine per minute at 25℃, pH 7.2
催化反應:
產品描述:Bovine plasma amine oxidase has a molecular weight of 170 kDa and an optimum pH of 6.2 for spermine and 7.2 for spermidine. Amine oxidases are divided into two classes: the pyridoxal and copper containing enzyme to which plasma amine oxidase belongs, and the FAD-containing amino oxidases. Natural substrates include catecholamines, tryptamine derivatives and other physiologically active amines. Plasma amine oxidase is used in research requiring nitrogen group transfers. The molecule is composed of two identical polypeptide chains. There are two pyridoxal phosphates and two atoms of Cu+ per molecule. Bovine plasma amine oxidase is inhibited by copper chelating agents, many carboxyl reagents such as cuprizone, hydroxylamine and cyanide. Benzoic acid and benzyl alcohol are both non-competitive inhibitors (KI = 30 and 34mM respectively). The assay for determination of amine oxidases employed at Worthington is essentially that of Tabor et al., JBC, 208, 645, (1954) with the reaction temperature reduced to 25℃
性狀(以下信息僅供參考):粉末。EC3.1.1.4
用途:本品僅供科研,不得用于其它用途
保存:-20℃
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