人腫瘤壞死因子β(TNF-β)ELISA Kit說(shuō)明書
elisa實(shí)驗(yàn)?zāi)康?br> 酶聯(lián)免疫吸附測(cè)定(enzyme-linked immunosorbent assay 簡(jiǎn)稱ELISA)是在免疫酶技術(shù)(immunoenzymatic techniques)的基礎(chǔ)上發(fā)展起來(lái)的一種新型的免疫測(cè)定技術(shù),ELISA過(guò)程包括抗原(抗體)吸附在固相載體上稱為包被,加待測(cè)抗體(抗原), 再加相應(yīng)酶標(biāo)記抗體(抗原),生成抗原(抗體)--待測(cè)抗體(抗原)--酶標(biāo)記抗體的復(fù)合物,再與該酶的底物反應(yīng)生成有色產(chǎn)物。借助分光光度計(jì)的光吸收計(jì)算抗體(抗原)的量。待測(cè)抗體(抗原)的定量與有色產(chǎn)生成正比。
人腫瘤壞死因子β(TNF-β)ELISA Kit說(shuō)明書
關(guān)于ELISA實(shí)驗(yàn)的知識(shí):
免疫吸附劑 已包被抗原或抗體的固相載體在低溫(2~8℃)干燥的條件下一般可保存6個(gè)月。有些不完整的試劑盒,僅供應(yīng)包被用抗原或抗體,檢測(cè)人員需自行包被。
人腫瘤壞死因子β(TNF-β)ELISA Kit說(shuō)明書
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