人白介素1(IL-1)ELISA Kit說(shuō)明書
Elisa實(shí)驗(yàn)原理:酶結(jié)合物與相應(yīng)抗原或抗體結(jié)合后,可根據(jù)加入底物的顏色反應(yīng)來(lái)判定是否有免疫反應(yīng)的存在,而且顏色反應(yīng)的深淺是與標(biāo)本中相應(yīng)抗原或抗體的量成正比例的,所以,可以按底物顯色的程度顯示試驗(yàn)結(jié)果。
人白介素1(IL-1)ELISA Kit說(shuō)明書
固相載體:固相載體在ELISA測(cè)定過(guò)程中作為吸附劑和容器,不參與化學(xué)反應(yīng)。可作ELISA中載體的材料很多,zui常用的是聚苯乙烯。聚苯乙烯具有較強(qiáng)的吸附蛋白質(zhì)的性能,抗體或蛋白質(zhì)抗原吸附其上后仍保留原來(lái)的免疫學(xué)活性,加之它的價(jià)格低廉,所以被普遍采用。聚苯乙烯為塑料,可制成各種形式。
人白介素1(IL-1)ELISA Kit說(shuō)明書
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