*E Fc段受體Ⅱ(FcεRⅡ/CD23)ELISA試劑盒
elisa實驗原理
用于免疫酶技術的酶有很多,如過氧化物酶,堿性磷酸酯酶,β-D-半乳糖苷酶,葡萄糖氧化酶,碳酸酐酶,乙酰*酯酶,6-磷酸葡萄糖脫氧酶等。常用于ELISA法的酶有辣根過氧化物酶,堿性磷酸酯酶等,其中尤以辣根過氧化物酶為多。由于酶摧化的是氧化還原反應,在呈色后須立刻測定,否則空氣中的氧化作用使顏色加深,無法準確地定量。
*E Fc段受體Ⅱ(FcεRⅡ/CD23)ELISA試劑盒
辣根過氧化物酶(HRP)是一種糖蛋白,每個分子含有一個氯化血紅素(protonhemin)區作輔基。酶的濃度和純度常以輔基的含量表示。氯化血紅素輔基的zui大吸收峰是403nm,HRP酶蛋白的zui大吸收峰是275nm,所以酶的濃度和純度計算式是(已知HRP的A(1cm 403nm 1%)=25,式中1%指HRP百分濃度為100ml含酶蛋白1g,即10mg/ml,所以,酶濃度以 mg/ml 計算是HRP的A(1cm 403nm mg/ml=2.5)HRP純度(RZ)=A403nm/A275nm純度RZ(Reinheit Zahl)值越大說明酶內所含雜質越少。高純度HRP的RZ值在3.0左右,zui高可達3.4。用于ELISA檢測的HRP的RZ值要求在3.0以上。
*E Fc段受體Ⅱ(FcεRⅡ/CD23)ELISA試劑盒
人原鈣黏素1(PCDH1)ELISA試劑盒 Human protocadherin 1,PCDH1 ELISA Kit
人白介素2受體(IL-2R)ELISA試劑盒 Human Interleukin-2 receptor,IL-2R ELISA Kit
人皮膚T細胞虜獲趨化因子(CTACK/CCL27)ELISA試劑盒 Human cutaneous T cell-attracting chemokine,CTACK ELISA Kit
人胸腎表達趨化因子(BRAK/CXCL14)ELISA試劑盒 Human Breast and kidney expressed chemokine,BRAK ELISA Kit
人B-淋巴細胞趨化因子1(BLC-1/CXCL13)ELISA試劑盒 Human B-Lymphocyte Chemoattractant 1,BLC-1 ELISA Kit
人結締組織活化肽Ⅲ(CTAPⅢ)ELISA試劑盒 Human connective tissue-activating peptide Ⅲ,CTAPⅢ ELISA Kit
人巨噬細胞集落刺激因子受體(M-CSFR)ELISA試劑盒 Human Macrophage Colony-Stimulating Factor Receptor,M-CSFR ELISA Kit
*A Fc段受體Ⅰ(FcαRⅠ/CD89)ELISA試劑盒 Human Receptor Ⅰ for the Fc region of immunoglobulin A,FcαRⅠ ELISA Kit
*E Fc段受體Ⅱ(FcεRⅡ/CD23)ELISA試劑盒 Human Receptor Ⅱ for the Fc region of immunoglobulin E,FcεRⅡ ELISA Kit
*G Fc段受體Ⅲ(FcγRⅢ/CD16)ELISA試劑盒 Human Receptor Ⅲ for the Fc region of immunoglobulin G,FcγRⅢ ELISA Kit
*G Fc段受體Ⅱ(FcγRⅡ/CD32)ELISA試劑盒 Human Receptor Ⅱ for the Fc region of immunoglobulin G,FcγRⅡ ELISA Kit
*G Fc段受體Ⅰ(FcγRⅠ/CD64)ELISA試劑盒G Fc段受體Ⅰ(FcγRⅠ/CD64)ELISA試劑盒 Human Receptor Ⅰ for the Fc region of immunoglobulin G,FcγRⅠ ELISA Kit
*G Fc段受體Ⅲ(FcγRⅢ/CD16)ELISA試劑盒 Human Receptor Ⅲ for the Fc region of immunoglobulin G,FcγRⅢ ELISA Kit
*G Fc段受體Ⅱ(FcγRⅡ/CD32)ELISA試劑盒 Human Receptor Ⅱ for the Fc region of immunoglobulin G,FcγRⅡ ELISA Kit
*G Fc段受體Ⅰ(FcγRⅠ/CD64)ELISA試劑盒 Human ReceptorⅠfor the Fc region of immunoglobulin G,FcγRⅠ ELISA Kit
人粒細胞趨化蛋白-2(GCP-2/CXCL6)ELISA試劑盒 Human granulocyte chemotactic protein-2,GCP-2 ELISA Kit
人ω干擾素(IFN-ω)ELISA試劑盒 Human Interferon ω,IFN-ω ELISA Kit
人糖基化依賴的細胞黏附分子(GlyCAM-1)ELISA試劑盒 Human glycosylation-dependent cell adhesion molecule-1,GlyCAM-1 ELISA Kit
人干擾素調節因子(IRF)ELISA試劑盒 Human interferon Regulatory Factor,IRF ELISA Kit
人淋巴毒素β(LTB)ELISA試劑盒 Human lymphotoxinβ,LTB ELISA Kit