人胸腎表達趨化因子(BRAK/CXCL14)ELISA試劑盒
Elisa實驗原理:抗原或抗體能以物理性地吸附于固相載體表面,可能是蛋白和聚苯乙烯表面間的疏水性部分相互吸附,并保持它的免疫學活性;
人胸腎表達趨化因子(BRAK/CXCL14)ELISA試劑盒
Elisa實驗常用方法:
雙抗體夾心法測定抗原
將抗原免疫*種動物獲得的抗體吸附于固相表面;
加抗原,形成抗原-抗體復合物;
加抗原免疫第二種動物獲得的抗體,形成抗體抗原抗體復合物;
加酶標抗抗體(第二種動物抗體的抗體);
加底物。底物的降解量=抗原量。
人胸腎表達趨化因子(BRAK/CXCL14)ELISA試劑盒
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