人抗巨細胞病毒抗體IgM(anti-CMV IgM) ELISA Kit代測
雙抗體夾心法測定抗原
將抗原免疫*種動物獲得的抗體吸附于固相表面;
加抗原,形成抗原-抗體復合物;
加抗原免疫第二種動物獲得的抗體,形成抗體抗原抗體復合物;
加酶標抗抗體(第二種動物抗體的抗體);
加底物。底物的降解量=抗原量。
人抗巨細胞病毒抗體IgM(anti-CMV IgM) ELISA Kit代測
Elisa實驗原理:酶結合物與相應抗原或抗體結合后,可根據加入底物的顏色反應來判定是否有免疫反應的存在,而且顏色反應的深淺是與標本中相應抗原或抗體的量成正比例的,所以,可以按底物顯色的程度顯示試驗結果。
人抗巨細胞病毒抗體IgM(anti-CMV IgM) ELISA Kit代測
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