人血小板型磷酸果糖激酶(PFKP)ELISA Kit說明書
ELISA試劑盒所需要樣本處理及要求 標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融.
不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
人血小板型磷酸果糖激酶(PFKP)ELISA Kit說明書
ELISA試劑盒所需要樣本處理及要求 組織標(biāo)本:切割標(biāo)本后,稱取重量。加入一定量的PBS,PH7.4。用液氮迅速冷凍保存?zhèn)溆谩?biāo)本融化后仍然保持2-8℃的溫度。加入一定量的PBS(PH7.4),用手工或勻漿器將標(biāo)本勻漿充分。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。分裝后一份待檢測,其余冷凍備用。
人血小板型磷酸果糖激酶(PFKP)ELISA Kit說明書
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