【CAS:9001-59-6,酸激酶,Pyruvate kinase,PK】
英文名稱:PK;Pyruvate Kinase from microorganism;ATP:pyruvate 2-O-phosphotransferase
CAS號(hào):9001-59-6
分子量:68KDa
級(jí)別:BR
活力:≥300units/mg protein
活力定義:1 U will convert one micromole of phosphoenolpyruvate to pyruvate per minute at pH 7.2 and 30 °C
pH stability:5.0~10.0(37℃,20hr)
Optimum PH:7.5
Thermal stability:≤60℃(pH8.5, 20min)
Optimum temperature:80°C
Preparation instructions:The enzyme is reconstituted in 50mM Tris-HC1 buffer,pH8.5 for activity assay
性狀:白色粉末,由4個(gè)亞基(分子量5500)組成。長(zhǎng)鏈脂肪酸、乙酰*、ATP和*對(duì)酶活有抑制作用,1,6-二磷酸果糖有活化作用。
用途:生化研究。酸激酶一種參與糖酵解途徑第10步反應(yīng)的激酶,是一個(gè)別構(gòu)酶。它催化磷酸烯醇式酸轉(zhuǎn)化而成,同時(shí)使ADP磷酸化生成ATP
保存:-20℃
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