細(xì)胞名稱

BHK-21 [C-13]倉(cāng)鼠腎成纖維細(xì)胞

描述

1961年3月，I.A. Macpherson和M.G.P. Stoker從1日齡的倉(cāng)鼠建立了BHK-21 (C-13)的親本。隨后84天連續(xù)培養(yǎng)，僅中斷8天進(jìn)行凍存，通過(guò)單細(xì)胞分離建立了13號(hào)克隆。這株細(xì)胞可用作轉(zhuǎn)染宿主，用于表達(dá)包含選擇及擴(kuò)增標(biāo)記的DNA(如Factor VIII,見ATCC CRL-8544)的載體。

動(dòng)物種別

倉(cāng)鼠

性別

***

組織來(lái)源

正常腎

形態(tài)

成纖維細(xì)胞

培養(yǎng)基和添加劑

MEM培養(yǎng)基(GIBCO,貨號(hào)41500034，添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，90%；優(yōu)質(zhì)胎牛血清，10%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。

供應(yīng)限制

REFERENCE

Gitschier J , et al. Genetic mapping and diagnosis of haemophilia A achieved through a BclI polymorphism in the factor VIII gene. Nature 314: 738-740, 1985. PubMed: 2986011 Hutz MH , et al. Restriction site polymorphism in the phosphoglycerate kinase gene on the X chromosome. Hum. Genet. 66: 217-219, 1984. PubMed: 6325324 Macpherson I , Stoker M . Polyoma transformation of hamster cell clones--an investigation of genetic factors affecting cell competence. Virology 16: 147-151, 1962. PubMed: 14468055 Virology 14: 359-370, 1961. Macpherson I . Characteristics of a hamster cell clone transformed by polyoma virus. J. Natl. Cancer Inst. 30: 795-815, 1963. Deleersnyder V , et al. Formation of native hepatitis C virus glycoprotein complexes. J. Virol. 71: 697-704, 1997. PubMed: 8985401 Yang TT , et al. Quantification of gene expression with a secreted alkaline phosphatase reporter system. BioTechniques 23: 1110-1114, 1997. PubMed: 9421645 Hussain MA , et al. POU domain transcription factor brain 4 confers pancreatic alpha-cell-specific expression of the proglucagon gene through interaction with a novel proximal promoter G1 element. Mol. Cell. Biol. 17: 7186-7194, 1997. PubMed: 9372951 You M , et al. ch-IAp1, a member of the inhibitor-of-apoptosis protein family, is a mediator of the antiapoptotic activity of the v-Rel oncoprotein. Mol. Cell. Biol. 17: 7328-7341, 1997. PubMed: 9372964 Jelachich ML , Lipton HL . Theiler's murine encephalomyelitis virus kills restrictive but not permissive cells by apoptosis. J. Virol. 70: 6856-6861, 1996. PubMed: 8794327 Schnell MJ , et al. The minimal conserved transcription stop-start signal promotes stable expression of a foreign gene in vesicular stomatitis virus. J. Virol. 70: 2318-2323, 1996. PubMed: 8642658 Chang YE , et al. Properties of the protein encoded by the UL32 open reading frame of herpes simplex virus 1. J. Virol. 70: 3938-3946, 1996. PubMed: 8648731 Biological evaluation of medical devices. Part 5: Tests for in vitro cytotoxicity. Sydney, NSW, Australia: Standards Australia; Standards Australia AS ISO 10993.5-2002. Biological evaluation of medical devices--Part 5: Tests for in vitro cytotoxicity. Geneva (Switzerland): International Organization for Standardization/ANSI; ISO ISO 10993-5:1999. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.