細(xì)胞名稱

MG-63人骨肉瘤細(xì)胞

描述

用聚*-聚胞嘧啶核苷酸，放線*酮和*可以誘導(dǎo)產(chǎn)生高水平的干擾素。

動(dòng)物種別

人

性別

男

組織來源

骨；骨肉瘤

形態(tài)

成纖維細(xì)胞

培養(yǎng)基和添加劑

MEM培養(yǎng)基(GIBCO,貨號(hào)41500034，添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，90%；優(yōu)質(zhì)熱滅活胎牛血清，10%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。

供應(yīng)限制

REFERENCE

Billiau A , et al. Human interferon: mass production in a newly established cell line, MG-63. Antimicrob. Agents Chemother. 12: 11-15, 1977. PubMed: 883813 Takeuchi Y , et al. Relationship between actions of transforming growth factor (TGF)-beta and cell surface expression of its receptors in clonal osteoblastic cells. J. Cell. Physiol. 162: 315-321, 1995. PubMed: 7860639 Yee A , et al. Biochemical characterization of the human cyclin-dependent protein kinase activating kinase. J. Biol. Chem. 271: 471-477, 1996. PubMed: 8550604