金屬蛋白酶組織抑制因子-4抗體 Anti-TIMP-4相關(guān)標記     Alexa Fluor 350   Alexa Fluor 488   Alexa Fluor 555   Alexa Fluor 647   AP  APC   Biotin   Cy3   Cy5  
 Cy5.5   Cy7   FITC   Gold   HRP   PE   PE-Cy3   PE-CY5   PE-CY5.5   PE-CY7  RBITC  
英文名稱     Anti-TIMP-4
中文名稱     金屬蛋白酶組織抑制因子-4抗體
別    名     Metalloproteinase inhibitor 4; TIMP 4; TIMP4; TIMP metallopeptidase inhibitor 4; TIMP-4; Timp4; TIMP4_HUMAN; Tissue inhibitor of metalloproteinase 4.
濃    度     1mg/1ml
抗體來源     Rabbit
克隆類型     polyclonal
交叉反應     Human, Mouse, Rat, Cow, Horse, Rabbit, 
產(chǎn)品類型     一抗  
研究領(lǐng)域     腫瘤 心血管 神經(jīng)生物學 合成與降解
蛋白分子量     predicted molecular weight: 22kDa
性    狀     Lyophilized or Liquid
免 疫 原     KLH conjugated synthetic peptide derived from human TIMP-4
亞    型     IgG
純化方法     affinity purified by Protein A
儲 存 液     0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
金屬蛋白酶組織抑制因子-4抗體 Anti-TIMP-4產(chǎn)品應用      WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 
（石蠟切片需做抗原修復） 

 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.
保存條件     Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note     This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹    The tissue inhibitors of metalloproteinases (TIMPs) are naturally occurring proteins that specifically inhibit matrix metalloproteinases and regulate extracellular matrix turnover and tissue remodeling by forming tightbinding inhibitory complexes with the MMPs. Thus, TIMPs maintain the balance between matrix destruction and formation. An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors. The TIMP proteins share several structural features including six loops held in place by six disulfide bonds arranged in three knot-like structures. These proteins also contain twelve cysteine residues in conserved regions of the molecule that form six disulfide bonds, essential for the formation of native conformations, and the N terminal region that is necessary for inhibitory activities. The N terminus of each TIMP contains a consensus sequence (VIRAK) and each TIMP is translated with a 29 amino acid leader sequence that is cleaved off to produce the mature protein. The C terminal regions are divergent, which enhances the selectivity of inhibition and binding efficiency. Although the TIMP proteins share high homology, they may either be secreted extracellularly in soluble form (TIMP1, TIMP2 and TIMP4) or bind to extracellular matrix components (TIMP3). 
The MMPs and TIMPs can be divided into two groups with respect to gene expression: the majority exhibit inducible expression and a small number are produced constitutively or are expressed at very low levels and are not inducible. Among agents that induce MMP and TIMP production are the inflammatory cytokines TNF alpha and IL1 beta. A marked cell type specificity is a hallmark of both MMP and TIMP gene expression (i.e. a limited number of cell types can be induced to make these proteins). Tissue Inhibitor of Metalloproteinases 4 (TIMP4) was identified by molecular cloning. TIMP4 shows 37 % amino acid identity with TIMP1 and 51 % homology with TIMP2 and TIMP3. TIMP4 is secreted extracellularly, predominantly in heart and brain tissue. It may function in a tissue specific fashion in extracellular matrix (ECM) homeostasis. TIMP4 has a strong inhibitory effect on the invasion of human breast cancer cells across reconstituted basement membranes suggesting that TIMP4 may have an important role in inhibiting primary tumor growth and progression. The human TIMP4 gene has the chromosomal location of 3p25. 
Function : Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.
Subcellular Location : Secreted.
Tissue Specificity : Abundant in heart and present at low levels in many other tissues. 
Similarity : Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
Database links : UniProtKB/Swiss-Prot: Q99727.1
合成與降解（Synthesis and Degradation）
TIMP-4是TIMP家族的新成員，TIMP-3與TIMP-4同樣具有較強的促凋亡、抑制血管生成、抗腫瘤浸潤作用。