CD10抗體相關標記     Alexa Fluor 350   Alexa Fluor 488   Alexa Fluor 555   Alexa Fluor 647   AP   APC   Biotin  Cy3   Cy5  
 Cy5.5   Cy7   FITC   Gold   HRP   PE   PE-Cy3   PE-CY5   PE-CY5.5   PE-CY7   RBITC  
英文名稱    CD10
中文名稱    CD10抗體
別    名    Atriopeptidase; CALLA; CD 10; CD10; Common acute lymphocytic leukemia antigen; DKFZp686O16152; EC 3.4.24.11; Enkephalinase; EPN; gp100; Membrane metallo endopeptidase; Membrane metalloendopeptidase; Membrane metalloendopeptidase neutral endopeptidase enkephalinase; Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10; Membrane metalloendopeptidase variant 1; Membrane metalloendopeptidase variant 2; MGC126681; MGC126707; MME; NEP; Neprilysin; Neutral endopeptidase 24.11; Neutral endopeptidase; NEP_HUMAN; Neprilysin; Skin fibroblast elastase; SFE.
研究領域    腫瘤  細胞膜受體  
抗體來源    Rabbit
克隆類型    一抗
交叉反應    Human, Mouse, Rat, 
產品應用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量    82kDa
細胞定位    細胞膜 
性    狀    Lyophilized or Liquid
濃    度    1mg/1ml
免 疫 原    KLH conjugated synthetic peptide derived from human CD10
亞    型    IgG
純化方法    affinity purified by Protein A
儲 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
保存條件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產品介紹    background:
This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing.

Function:
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.

Subcellular Location:
Cell membrane; Single-pass type II membrane protein.

Post-translational modifications:
Myristoylation is a determinant of membrane targeting. 
Glycosylation at Asn-628 is necessary both for surface expression and neutral endopeptidase activity.

Similarity:
Belongs to the peptidase M13 family.

Gene ID:
4311

Database links:
Entrez Gene: 4311 Human
Entrez Gene: 17380 Mouse
Entrez Gene: 24590 Rat
Omim: 120520 Human
SwissProt: P08473 Human
SwissProt: Q61391 Mouse
SwissProt: P07861 Rat
Unigene: 307734 Human
Unigene: 296022 Mouse
Unigene: 33598 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

CD10即共同型急性淋巴細胞白血病抗原,是II型跨膜糖蛋白.CD10表達于早期B淋巴細胞,某些粒性白細胞,骨髓基質細胞,部分上皮組織及其起源的腫瘤細胞中也有表達.主要應用于某些惡性淋巴瘤和白血病的分型.CD10是一種94kD的鋅依賴性細胞膜金屬結合蛋白，參與細胞神經肽和激素肽的后分泌過程。它廣泛地分布于腎、肝、小腸、胎盤、脈絡膜叢、腦腺、腺皮質和白細胞，CD10是生發中心細胞及所致淋巴瘤的標志物。