小鼠單核細(xì)胞趨化蛋白4(MCP-4/CCL13)ELISA試劑盒運輸方法：泡沫箱配生物冰袋運輸
價格及詳細(xì)資料：電議,或咨詢在線客服,或者以郵件形式發(fā)到我司qysw@qiyibio.com .齊一生物科技（上海）有限公司提供ELISA試劑盒受到了廣大科研單位*肯定和認(rèn)同。*保證，價格公道，傾力為國內(nèi)外科研院校實驗室提供產(chǎn)品。若有需要，我司將竭誠為您服務(wù)！
FOR RESEARCH USE ONLY
Drug Names
Generic Name：小鼠單核細(xì)胞趨化蛋白4(MCP-4/CCL13)ELISA試劑盒
This kit can be used for determination of serum, plasma and liquid samples Organization Content.
The experimental principle:
The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.
Materials provided with the kit
Materials provided with the kit    48determinations    96 determinations    Storage
User manual    1    1    
Closure plate membrane    2    2    
Sealed bags    1    1    
Microelisa stripplate    1    1    2-8℃
Standard：360ng/L    0.5ml×1 bottle    0.5ml×1 bottle    2-8℃
Standard diluent    1.5ml×1 bottle    1.5ml×1 bottle    2-8℃
HRP-Conjugate reagent    3ml×1 bottle    6ml×1 bottle    2-8℃
Sample diluent    3ml×1 bottle    6ml×1 bottle    2-8℃
Chromogen Solution A    3ml×1 bottle    6ml×1 bottle    2-8℃
Chromogen Solution B    3ml×1 bottle    6ml×1 bottle    2-8℃
Stop Solution    3ml×1 bottle    6ml×1 bottle    2-8℃
wash  solution    （20ml×20 fold）
×1bottle    （20ml×30 fold）
×1bottle    2-8℃
Specimen requirements
1.serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
2.plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
3.Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.
4.cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, 小鼠單核細(xì)胞趨化蛋白4(MCP-4/CCL13)ELISA試劑盒Centrifugal again.
5.Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.
6.extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
7.Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1.Dilute and add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first and the second well, then add Standard dilution 50μl to the first and the second well, mix; take out 100μl form the first and the second well then add it to the third and the forth well separay. then add Standard dilution 50μl to the third and the forth well ,mix ; then take out 50μl from the third and the forth well discard, add 50μl to the fifth and the sixth well ,then add Standard dilution 50μl to the fifth and the sixth well, mix ; take out 50μl from the fifth and the sixth well and add to the seventh and the eighth well, then add Standard dilution 50μl to the seventh and the eighth well ,mix ; take out 50μl from the seventh and the eighth well and add to the ninth and the tenth well, add Standard dilution 50μl to the ninth and the tenth well, mix , take out 50μl from the ninth and the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)
2.add sample：Set blank wells separay (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well. 
7.incubate：Operation with 3.
8.washing：Operation with 5.
9.color：Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
Important notes
1.The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2.washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3.add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley .
4.if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.（×n×5）.
5.Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6.The substrate evade the light preservation.
7.Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8.All samples, washing buffer and each kind of reject should according to infective material process.
9.Do not mix reagents with those from other lots.
Calculate：
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
Storage and validity
1．Storage：  2-8℃.
2．validity： six months.

小鼠單核細(xì)胞趨化蛋白4(MCP-4/CCL13)ELISA試劑盒其它產(chǎn)品PCB, Turntable, X-Main    9016299    qiagen    德國    齊一生物    見說明
Holder, tip rack, unit 6-fold    9011900    qiagen    德國    齊一生物    見說明
Handle, HS shaker adapter plate, BR8000    9011779    qiagen    德國    齊一生物    見說明
PyroMark Q96 MD Software (5)    9019086    qiagen    德國    齊一生物    見說明
PyroMark CpG SW 1.0 (5)    9019068    qiagen    德國    齊一生物    見說明
User Manual, EZ1xl    9019031    qiagen    德國    齊一生物    見說明
Damper, door, EZ1xl    9019021    qiagen    德國    齊一生物    見說明
Grid, UV lamp, EZ1xl    9019004    qiagen    德國    齊一生物    見說明
Hinge, for door, EZ1xl    9019003    qiagen    德國    齊一生物    見說明
RBlock, 16x0.2ml PCR 8x2ml/1.5ml Flat/Ta    9018951    qiagen    德國    齊一生物    見說明
Adapter, 33x2ml flat base Tubes    9018930    qiagen    德國    齊一生物    見說明
Rotor-Gene AssayManager SOW(10 licenses)    9022739    qiagen    德國    齊一生物    見說明
Retainer, PMT Baffle, DML3000    9022707    qiagen    德國    齊一生物    見說明
Extension set (Z), 4 x 50mm, Pos. Sys.    9016027    qiagen    德國    齊一生物    見說明
Connecting Tool, Disp.Head, BR8 V2.0    9015911    qiagen    德國    齊一生物    見說明
EZ1 FNSC Card    9015864    qiagen    德國    齊一生物    見說明
AC fuse, 115 V, 250 8A, M48    9015638    qiagen    德國    齊一生物    見說明
Window, for door    9015537    qiagen    德國    齊一生物    見說明
PCB 2 - Connection, PyroQ96MD    9019170    qiagen    德國    齊一生物    見說明
Cog belt kit - X/Y, PyroQ96MD    9019157    qiagen    德國    齊一生物    見說明
Rubber spring (10), Microplate Holder LH    9014145    qiagen    德國    齊一生物    見說明
PC, DCU    9021254    qiagen    德國    齊一生物    見說明
Power Supply Module 1 (U164), DCU    9021135    qiagen    德國    齊一生物    見說明
Valve Manifold, Gripper, DCU    9021121    qiagen    德國    齊一生物    見說明
Controller, Theta, DCU    9021054    qiagen    德國    齊一生物    見說明
Frame, transfer module, Qsym    9021031    qiagen    德國    齊一生物    見說明
Lid, waste box, AS, Qsym    9021024    qiagen    德國    齊一生物    見說明
Notebook, small instruments    9020962    qiagen    德國    齊一生物    見說明
Cooling adapter, MTP, RB, v2, Qsym    9020729    qiagen    德國    齊一生物    見說明
Fan, electrical, 80mm, C34    9020710    qiagen    德國    齊一生物    見說明
Table plate, complete, C12    9020702    qiagen    德國    齊一生物    見說明
Microplate Station 8000, tall    9016654    qiagen    德國    齊一生物    見說明
Plate Stacker, RCS1    9016584    qiagen    德國    齊一生物    見說明
Bucket  Set (2), for microplate, QNG    9016521    qiagen    德國    齊一生物    見說明
Adapter PCB, Gateway, Kloehn    9016483    qiagen    德國    齊一生物    見說明
EZ1 DNA Investigator Card    9016387    qiagen    德國    齊一生物    見說明
TFT Monitor, 17"    9016308    qiagen    德國    齊一生物    見說明
Software QIAsoft M48    9016172    qiagen    德國    齊一生物    見說明
Lens array, PyroQ96MD    9019175    qiagen    德國    齊一生物    見說明
Holder, bottle 30ml    9011925    qiagen    德國    齊一生物    見說明
Tubing, teflon, M6, 3 x 0.5 mm, 0.1 m    9011891    qiagen    德國    齊一生物    見說明
Shaker, Tube Rack for Plato 3    9011872    qiagen    德國    齊一生物    見說明
Cooling/heating unit, BR8000 v1.01    9011860    qiagen    德國    齊一生物    見說明
Elution Microplate Adapter 3000, SBS    9236850    qiagen    德國    齊一生物    見說明