小鼠金屬蛋白酶組織抑制因子-1(TIMP-1)ELISA試劑盒FOR RESEARCH USE ONLY
Drug Names
Generic Name：小鼠金屬蛋白酶組織抑制因子-1(TIMP-1)ELISA試劑盒
This kit can be used for determination of serum, plasma and liquid samples Organization Content.
The experimental principle:
The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.
Materials provided with the kit
Materials provided with the kit    48determinations    96 determinations    Storage
User manual    1    1    
Closure plate membrane    2    2    
Sealed bags    1    1    
Microelisa stripplate    1    1    2-8℃
Standard：360ng/L    0.5ml×1 bottle    0.5ml×1 bottle    2-8℃
Standard diluent    1.5ml×1 bottle    1.5ml×1 bottle    2-8℃
HRP-Conjugate reagent    3ml×1 bottle    6ml×1 bottle    2-8℃
Sample diluent    3ml×1 bottle    6ml×1 bottle    2-8℃
Chromogen Solution A    3ml×1 bottle    6ml×1 bottle    2-8℃
Chromogen Solution B    3ml×1 bottle    6ml×1 bottle    2-8℃
Stop Solution    3ml×1 bottle    6ml×1 bottle    2-8℃
wash  solution    （20ml×20 fold）
×1bottle    （20ml×30 fold）
×1bottle    2-8℃
Specimen requirements
1.serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
2.plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
3.Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.
4.cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
5.Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.
6.extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
7.Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1.Dilute and add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first and the second well, then add Standard dilution 50μl to the first and the second well, mix; take out 100μl form the first and the second well then add it to the third and the forth well separay. then add Standard dilution 50μl to the third and the forth well ,mix ; then take out 50μl from the third and the forth well discard, add 50μl to the fifth and the sixth well ,then add Standard dilution 50μl to the fifth and the sixth well, mix ; take out 50μl from the fifth and the sixth well and add to the seventh and the eighth well, then add Standard dilution 50μl to the seventh and the eighth well ,mix ; take out 50μl from the seventh and the eighth well and add to the ninth and the tenth well, add Standard dilution 50μl to the ninth and the tenth well, mix , take out 50μl from the ninth and the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)
2.add sample：Set blank wells separay (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well. 
7.incubate：Operation with 3.
8.washing：Operation with 5.
9.color：Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
Important notes
1.The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2.washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3.add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley .
4.if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.（×n×5）.
5.Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6.The substrate evade the light preservation.
7.Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8.All samples, washing buffer and each kind of reject should according to infective material process.
9.Do not mix reagents with those from other lots.
Calculate：
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
小鼠金屬蛋白酶組織抑制因子-1(TIMP-1)ELISA試劑盒Storage and validity
1．Storage：  2-8℃.
2．validity： six months.

其它產(chǎn)品9019561    Bracket, X-axis opto, Cxx        740.00     CNY
9019539    Power entry port, Cxx        877.00     CNY
9019523    BLOCK LOADING 80 WELL COM ASSY        3,410.00     CNY
9019507    RISER VACUUM 20MM        3,550.00     CNY
216213    FastLane Cell SYBR Green Kit (200)        21,610.00     CNY
204076    Rotor-Gene SYBR Green PCR Kit (2000)        15,890.00     CNY
9019405    Board, distribution incl. PTCs, RG3        1,120.00     CNY
9019388    LED, green (530), RG3        937.00     CNY
9019359    Filter, fuse, switch mains power, RGx        1,290.00     CNY
9019344    Sensor, optics source home, RG6        440.00     CNY
9019342    LED, IR, RG6        670.00     CNY
9234701    Mikrowin Kinetic Software        57,750.00     CNY
9020470    Cover, front, GSC        15,220.00     CNY
9234663    Fuse T6,3A        612.00     CNY
1027284    AllStars Neg. siRNA AF 488 (5 nmol)        2,060.00     CNY
1027272    miScript Negative Control Inhititor(20)        11,160.00     CNY
9233431    Belt, tooth, handler z, BR8000        612.00     CNY
9019232    Opto - Y, PyroQ24        3,250.00     CNY
9019212    Robot complete, PyroQ96MD        214,110.00     CNY
9019201    PCB - Gripper, PyroQ96MD        5,270.00     CNY
1021055    Protease Solvent + Azid (6/15),KG        368.00     CNY
1018420    Taq DNA Polymerase 25,000 units/vial    OEM Product    104,230.00     CNY
1064143    DNase Booster (2/2), KG        406.00     CNY
1061086    TB Quantification Standards        97.00     CNY
1061090    HIV-1 Quantification Standards        97.00     CNY
1061093    HCV Controls & Quantification Standards        97.00     CNY
1061099    HCMV PCR REAGENT II        1,880.00     CNY
1061118    HBV PCR REAGENT IV        2,070.00     CNY
9232897    Twin Head (Tandem) Washer Pump (Vaccuum)        42,140.00     CNY
9016686    Washer Boat, RCS1        19,530.00     CNY
9016641    Repair Kit, DISU, pump, BR8, v2.0        1,930.00     CNY
9016600    Tip-Tray Holder, 50 µl tips        7,880.00     CNY
9019758    Interlock jumper (2), V2, QX        265.00     CNY
9013830    Front Reagent Rack 1.03        16,090.00     CNY
9013508    Gel-tray adapter, large        3,860.00     CNY
9019622    Cable, P assembly, 1ch., Cx2        1,560.00     CNY
9019593    Belt, Z-axis gearbox (grey), 8 ch., Cx8        290.00     CNY
9019591    O-ring, sink separator, CX1        127.00     CNY
9019578    Cable, Earthlink 85 mm, Cxx        196.00     CNY
9019563    Bracket, mounting Y-axis, Cxx        2,980.00     CNY
9019542    Gas Strut, lid, 145 N, CQx        612.00     CNY
9019509    Pinch Valve, C18        12,710.00     CNY
9019435    Cover        9,780.00     CNY
9019421    Set, fuses, 5A (6), 0.8A (6), RG3        555.00     CNY
9019390    LED, yellow (585), RG3        937.00     CNY
9019372    Hinge assembly, rear hinge plate, RG6        1,720.00     CNY
9019357    Transformer, heater, RG6        3,690.00     CNY
1027418    Flexitube siRNA 20 nmol w/o Modification        3,850.00     CNY
1027295    AllStars Neg. siRNA AF 647 (20 nmol)        3,640.00     CNY
9019325    Kit, heater FETw/drivers, RG6        1,230.00     CNY
9019234    Gasket - Cartridge, PyroQ24        5,270.00     CNY
9234760    Manual, user, hardware BR3000 int        5,050.00     CNY
9234735    Manual, user, hardware BR3000 US        5,050.00     CNY
9234703    Mikrowin data reduction software, upg.        46,180.00     CNY
9234696    Manual Hardware Plato 1/3/4        1,780.00     CNY
204072    Rotor-Gene SYBR Green PCR Kit (80)    The Rotor-Gene SYBR Green PCR Kit is dedicated for use with the Rotor-Gene Q cycler, providing ultrafast, highly specific quantification in real-time PCR and two-step RT-PCR using SYBR Green I.    944.00     CNY
9020471    Spacer, 0.2 mm long set, GSC        937.00     CNY
9233721    Rack for Biovue Cards (holds 6 cards)        5,050.00     CNY
9233456    Ring, centring, pressure sensor        612.00     CNY
9019186    Cable - Pressure, PyroQ96MD        1,430.00     CNY
9019203    Opto - Robot Y motor , PyroQ96MD        2,020.00     CNY
1022070    Luciferase GL2 siRNA (5 nm)        1,480.00     CNY
1020460    Top Elute Fluid 48 x (1,48/1,5),KG        4,080.00     CNY
1018013    Buffer RPE (55/250) N,KG        440.00     CNY
1014636    Buffer G2 (260/250),KG        867.00     CNY
1066847    PG NDV Univ. RT-PCR Assay (48) LE Z        3,260.00     CNY
1061108    AIV-H9 RT-PCR REAGENT        3,260.00     CNY
9016623    BR MDx CTM 96 PCR Setup, PRO        1,930.00     CNY
9016587    Microplte Washer, RCS1        98,100.00     CNY
9016515    Bucket Set (2), for microplate, sealable        23,400.00     CNY
9019856    36-well High Profile Rotor        3,610.00     CNY
9019854    72-well High Profile Rotor        3,490.00     CNY
9019835    Service Kit - X/Y, PyroQ96ID        31,240.00     CNY
9019804    ZLV Upgrade Package 2, RCS        65,880.00     CNY
9019766    Sensor, Y, with cable, V2, QX        694.00     CNY
9019757    Anti-rotation tool, V2, QX        808.00     CNY
9013738    Y/Z Electr ASMBL, RCS1        49,790.00     CNY
9013438    Quarterly maintenance kit BR 96O4        3,240.00     CNY
9013432    Maintenance kit, semi annual, BR9600 (F)        6,510.00     CNY
9013424    Maintenance Kit BR 9600 (E)        46,180.00     CNY
9238804    Clip for sealing cap        346.00     CNY
9019675    Notebook, XP        27,540.00     CNY
9019667    PCB - Heat Lid, PyroQ24        3,410.00     CNY
9019660    Torx key set, PyroQ96VW        290.00     CNY
9019646    Mixer module, PyroQ96ID        35,520.00     CNY
9019636    Board, power branching, EZ1xl        16,300.00     CNY
9019617    Pipettor, vacuum hub assembly, Cx2        5,380.00     CNY
9019596    Tee, nylon barb, 10 mm, CX1        196.00     CNY
9019566    Clamp, Y-arm mounting, Cxx        196.00     CNY
9019553    Rail, guide 314mm Y-axis, CASx        10,250.00     CNY
9019530    HEPA filter system, QIA        26,610.00     CNY
9019511    Sink, vac station, C18        5,430.00     CNY
9019504    CARRIAGE TRAN HIGH SKIRT 1820        6,650.00     CNY
9019434    Top plate heater assembly        7,460.00     CNY
9019392    Filter, air, RGx        740.00     CNY
9019387    LED, red (625), RG3        937.00     CNY
9019374    Housing, bearing w/coupling, motor, RG6        11,330.00     CNY
9019362    Connector USB, serial internalcabl, RG6        555.00     CNY
9019353    Flap, cooling, RG6        2,020.00     CNY
1027411    FlexiPlate siRNA - 0.1 nmol        265.00     CNY
1027310    Negative Control siRNA (20 nmol)        2,630.00     CNY
1027298    AllStars Cell Death Contr.siRNA (5nmol)        1,480.00     CNY
9019341    LED, yellow (585), RG6        335.00     CNY
9234712    Board, level sensor, BR8000 v1.02        3,720.00     CNY
9020469    Peltier module, GSC        1,140.00     CNY
9234541    Pumphead click'n'go; HSPS; BR3/9604        43,590.00     CNY