小鼠腫瘤壞死因子α(TNF-α)ELISA試劑盒價格：電議,或咨詢在線客服,或者以郵件形式發到我司qysw@qiyibio.com .齊一生物科技（上海）有限公司提供的試劑盒受到了廣大科研單位的*肯定和認同。*保證，價格公道，傾力為國內外科研院校實驗室提供的產品。若有需要，我司將竭誠為您服務！

FOR RESEARCH USE ONLY
Drug Names
Generic Name：小鼠腫瘤壞死因子α(TNF-α)ELISA試劑盒
This kit can be used for determination of serum, plasma and liquid samples Organization Content.
The experimental principle:
The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.
Materials provided with the kit
Materials provided with the kit    48determinations    96 determinations    Storage
User manual    1    1    
Closure plate membrane    2    2    
Sealed bags    1    1    
Microelisa stripplate    1    1    2-8℃
Standard：360ng/L    0.5ml×1 bottle    0.5ml×1 bottle    2-8℃
Standard diluent    1.5ml×1 bottle    1.5ml×1 bottle    2-8℃
HRP-Conjugate reagent    3ml×1 bottle    6ml×1 bottle    2-8℃
Sample diluent    3ml×1 bottle    6ml×1 bottle    2-8℃
Chromogen Solution A    3ml×1 bottle    6ml×1 bottle    2-8℃
Chromogen Solution B    3ml×1 bottle    6ml×1 bottle    2-8℃
Stop Solution    3ml×1 bottle    6ml×1 bottle    2-8℃
wash  solution    （20ml×20 fold）
×1bottle    （20ml×30 fold）
×1bottle    2-8℃
Specimen requirements
1.serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
2.plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
3.Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.
4.cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
5.Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.
6.extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
7.Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1.Dilute and add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first and the second well, then add Standard dilution 50μl to the first and the second well, mix; take out 100μl form the first and the second well then add it to the third and the forth well separay. then add Standard dilution 50μl to the third and the forth well ,mix ; then take out 50μl from the third and the forth well discard, add 50μl to the fifth and the sixth well ,then add Standard dilution 50μl to the fifth and the sixth well, mix ; take out 50μl from the fifth and the sixth well and add to the seventh and the eighth well, then add Standard dilution 50μl to the seventh and the eighth well ,mix ; take out 50μl from the seventh and the eighth well and add to the ninth and the tenth well, add Standard dilution 50μl to the ninth and the tenth well, mix , take out 50μl from the ninth and the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)
2.add sample：Set blank wells separay (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well. 
7.incubate：Operation with 3.
8.washing：Operation with 5.
9.color：Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
Important notes
1.The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2.washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3.add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley .
4.if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.（×n×5）.
5.Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6.The substrate evade the light preservation.
7.Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8.All samples, washing buffer and each kind of reject should according to infective material process.
9.Do not mix reagents with those from other lots.
小鼠腫瘤壞死因子α(TNF-α)ELISA試劑盒Calculate：
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
Storage and validity
1．Storage：  2-8℃.
2．validity： six months.

其它進口產品218073    miScript SYBR Green PCR Kit (200)
218075    miScript SYBR Green PCR Kit (1000)
218411    Human miScript Assay 96 Set (100)
218412    Mouse miScript Assay 96 Set (100)
218413    Rat miScript Assay 96 Set (100)
218421    Human miScript Assay 96 Set (20)
218422    Mouse miScript Assay 96 Set (20)
218423    Rat miScript Assay 96 Set (20)
218431    Human miScript Assay 384 Set (20)
218432    Mouse miScript Assay 384 Set (20)
218433    Rat miScript Assay 384 Set (20)
231122    QIAGEN PCR Cloning Kit (10)
231124    QIAGEN PCR Cloning Kit (40)
231222    QIAGEN PCR Cloningplus Kit (10)
231224    QIAGEN PCR Cloningplus Kit (40)
239025    GelPilot 50 bp Ladder (100)
239035    GelPilot 100 bp Ladder (100)
239045    GelPilot 100 bp Plus Ladder (100)
239085    GelPilot 1 kb Ladder (100)
239095    GelPilot 1 kb Plus Ladder (100)
239125    GelPilot Wide Range Ladder (100)
239135    GelPilot Mid Range Ladder (100)
239145    GelPilot High Range Ladder (100)
239901    GelPilot DNA Loading Dye, 5x
301005    Attractene Transfection Reagent (1 ml)
301007    Attractene Transfection Reagent (4 x 1 ml)
301105    PolyFect Transfection Reagent (1 ml)
301107    PolyFect Transfection Reagent (4 x 1 ml)
301108    PolyFect Transfection Reagent (100 ml)
301305    SuperFect Transfection Reagent (1.2 ml)
301307    SuperFect Transfection Reagent (4 x 1.2 ml)
301425    Effectene Transfection Reagent (1 ml)
301427    Effectene Transfection Reagent (4 x 1 ml)
301525    TransMessenger Transfection Reagent (0.5 ml)
301704    HiPerFect Transfection Reagent (0.5 ml)
301705    HiPerFect Transfection Reagent (1 ml)
301707    HiPerFect Transfection Reagent (4 x 1 ml)
301709    HiPerFect Transfection Reagent (100 ml)
301799    RNAi Human/Mouse Starter Kit
311802    BioMag Protein A (10 ml)
311812    BioMag Protein G (10 ml)
761115    PAXgene Blood DNA Tubes (100)
761133    PAXgene Blood DNA Kit (25)
762164    PAXgene Blood RNA Kit (50)
762174    PAXgene Blood RNA Kit (50)
762331    PAXgene 96 Blood RNA Kit (4)
762431    PAXgene Blood RNA MDx Kit (4)
762535    QIAsymphony PAXgene Blood RNA Kit (96)
763134    PAXgene Blood miRNA Kit (50)
764114    PAXgene Bone Marrow RNA Tube (50)
764133    PAXgene Bone Marrow RNA Kit (30)
765112    PAXgene Tissue Containers (10)
765134    PAXgene Tissue RNA Kit (50)
766134    PAXgene Tissue miRNA Kit (50)
767134    PAXgene Tissue DNA Kit (50)
805923    REPLI-g Service, Single Tube (100 µg)
805925    REPLI-g Service, Single Tube (500 µg)
805943    REPLI-g Service (100 µg)
805945    REPLI-g Service (500 µg)
820099    Plasmid Prep Service Customized
922902    LiquiChip System Fluid
922911    LiquiChip Calibration Bead Kit
922912    LiquiChip Control Bead Kit
929002    QIAxcel DNA High Resolution Kit (1200)
929004    QIAxcel DNA Screening Kit (2400)