當前位置:齊一生物科技(上海)有限公司>>ELISA試劑盒>>農殘類ELISA試劑盒>> 群勃龍檢測試劑盒
群勃龍檢測試劑盒規 格:96T
檢測標本:各種動物肌肉組織、肝臟、尿液、血清、水產、蜜蜂、牛奶、糧食作物(玉米、大米、豆類、花生、麥類等)、飼料等
檢測方法:ELISA
檢測類型:酶聯免疫分析法
產品的用途:僅供科研究課題使用
價格及詳細資料:電議,或咨詢在線客服,或者以郵件形式發到我司qysw@qiyibio.com .齊一生物科技(上海)有限公司提供的ELISA試劑盒受到了廣大科研單位的*肯定和認同。*保證,價格公道,傾力為國內外科研院校實驗室提供的產品。若有需要,我司將竭誠為您服務!
群勃龍檢測試劑盒本試劑盒用于測定各種動物肌肉組織、肝臟、尿液、血清、飼料等水產/蜂蜜/牛奶相關液體樣本中殘留的定量檢測。
實驗原理:
本試劑盒應用雙抗體夾心法測定標本中該產品水平。用純化的本產品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產品抗原,再與HRP標記的本產品抗體結合,形成抗體-抗原-酶標抗體復合物,經過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉化成藍色,并在酸的作用下轉化成zui終的黃色。顏色的深淺和樣品中的本產品呈正相關。用酶標儀在450nm波長下測定吸光度(OD值),通過標準曲線計算樣品中該產品濃度。
試劑盒組成:
試劑盒組成 | 48孔配置 | 96孔配置 | 保存 |
說明書 | 1份 | 1份 |
|
封板膜 | 2片(48) | 2片(96) |
|
密封袋 | 1個 | 1個 |
|
酶標包被板 | 1×48 | 1×96 | 2-8℃保存 |
標準品:1800ng/L | 0.5ml×1瓶 | 0.5ml×1瓶 | 2-8℃保存 |
標準品稀釋液 | 1.5ml×1瓶 | 1.5ml×1瓶 | 2-8℃保存 |
酶標試劑 | 3 ml×1瓶 | 6 ml×1瓶 | 2-8℃保存 |
樣品稀釋液 | 3 ml×1瓶 | 6 ml×1瓶 | 2-8℃保存 |
顯色劑A液 | 3 ml×1瓶 | 6 ml×1瓶 | 2-8℃保存 |
顯色劑B液 | 3 ml×1瓶 | 6 ml×1瓶 | 2-8℃保存 |
終止液 | 3ml×1瓶 | 6ml×1瓶 | 2-8℃保存 |
濃縮洗滌液 | (20ml×20倍)×1瓶 | (20ml×30倍)×1瓶 | 2-8℃保存 |
標本要求:
1.標本采集后盡早進行提取,提取按相關文獻進行,提取后應盡快進行實驗。若不能馬上進行試驗,可將標本放于-20℃保存,但應避免反復凍融
2.不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
操作步驟:
注意事項:
10. 如與英文說明書有異,以英文說明書為準。
計算:
以標準物的濃度為橫坐標,OD值為縱坐標,
在坐標紙上繪出標準曲線,根據樣品的OD
值由標準曲線查出相應的濃度;再乘以稀釋
倍數;或用標準物的濃度與OD值計算出標
準曲線的直線回歸方程式,將樣品的OD值
代入方程式,計算出樣品濃度,再乘以稀釋
倍數,即為樣品的實際濃度。
試劑盒性能:
1.樣品線性回歸與預期濃度相關系數R值為0.95以上。
2.批內與批見應分別小于9%和11%
保存條件及有效期:
1.試劑盒保存;2-8℃。
2.有效期:6個月
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