HT-29細胞人結腸腺癌細胞此細胞為我公司向ATCC細胞庫引進,細胞來源真實可靠。詳細說明書,請向客服索要,咨詢、定購。齊一生物科技(上海)有限公司:021- 60348496。Web:www.qiyibio.com
HT-29細胞人結腸腺癌細胞此細胞為我公司向ATCC細胞庫引進,細胞來源真實可靠。詳細說明書,請向客服索要,咨詢、定購。齊一生物科技(上海)有限公司:021- 60348496。Web:www.qiyibio。。com
HT-29細胞人結腸腺癌細胞一、細胞操作參考書
二、生長特性:貼壁 懸浮
三、細胞生長條件:
培養基 90% (GIBCO)
血清 10% FBS(GIBCO)
溫度 37℃
空氣條件 5% CO2,,95% AIR
生長代數 P4
凍存條件 培養基70%、血清20%、DMSO10%
四、組成:
組份 規格
細胞一瓶 T25
細胞培養與操作說明 1份
- 如果細胞為貼壁細胞,,而收到時呈懸浮或者部分懸浮的狀態,請將懸浮的細胞即時離心,加15%血清的*培養基到新的培養皿/瓶繼續培養3天;同時原培養瓶中剩下的貼壁細胞更換為15%血清的*培養基,培養3天。3天后若原瓶或者新瓶中的細胞都沒有出現增殖而是繼續脫落死亡,請及時實驗室,技術人員會跟進解決
- 貼壁細胞生長緩慢:適當提高血清濃度(zui高不能超過20%),或可根據該細胞生長密度,考慮胰酶消化后,轉移到新的培養瓶繼續培養
- 生長不均:貼壁細胞若出現分布不均,成島狀生長,可將細胞進行消化,重懸打散細胞,加入新鮮培養基進行培養
- 吸出原培養瓶中的培養基,PBS緩沖液潤洗細胞兩次,加2~3 ml 0.25%胰酶進行消化細胞(注意把握消化時間,通常控制在1-2min)
- 鏡下觀察消化情況,在細胞邊緣縮小,貼壁松動時(不建議消化到細胞漂浮)去掉胰酶,加6~8ml *培養基,輕輕吹打細胞層,盡量把細胞層吹落,吹散
- 取部分細胞懸液轉移到新的培養皿/瓶中,添加適當的*培養基,,把細胞懸液打勻,于培養箱中培養
- 注意培養基PH值變化情況,定期換液(每周2-3次),待細胞密度達到80%以后重復1項操作或者凍存
- Human Leptospira IgG,Lep IgG ELISA試劑盒
Human Amebiasis ELISA試劑盒
Human Diphtheria antibody ELISA試劑盒
Human Cysticercosis antybody,CYT Ab ELISA試劑盒
Human Brucella Antibody IgG,Brucella Ab IgG ELISA試劑盒
Human Varicella zoster virus IgM,VZV IgM ELISA試劑盒
Human Varicella zoster virus IgG,VZV-IgG ELISA試劑盒
Human Legionella antibody IgA,LP Ab-IgA ELISA試劑盒
Human Legionella antibody IgM,LP Ab-IgM ELISA試劑盒
Human Legionella antibody IgG,LP Ab-IgG ELISA試劑盒
Human paramyxovirus parotitis IgM ELISA試劑盒
Human paramyxovirus parotitis IgG ELISA試劑盒
Human epstein-barr virus IgM,EBv IgM ELISA試劑盒
Human epstein-barr virus IgA,EBv IgA ELISA試劑盒
Human epstein-barr virus IgG,EBv IgG ELISA試劑盒
Human Leishimaria antibody ELISA試劑盒
Human dengue fever antibody,DF ELISA試劑盒
Human Influenza viruses IgG,FLU ELISA試劑盒
Human Adenovirus IgM,ADV-IgM ELISA試劑盒
Human Adenovirus IgG,ADV-IgG ELISA試劑盒
Human Rotavirus,RV IgM ELISA試劑盒
human EchoVirus IgM,ECHO IgM ELISA試劑盒
Human anti-respiratory syncytial virus antibody,RSV ELISA試劑盒
Human measlesvirus IgM,MV IgM ELISA試劑盒
Human Coxsackie virus IgM,Cox V-IgM ELISA試劑盒
Human Coxsackie virus IgG,Cox V-IgG ELISA試劑盒
Human tuberculosis antibody IgG,TB-Ab IgG ELISA試劑盒
Human cicatricial Pemphigoid,CP ELISA試劑盒
human hepatitis B virus,HBV preS2Ab ELISA試劑盒
human hepatitis B virus,HBV preS2Ag ELISA試劑盒
Human Hepatitis G virus IgG,HGV-IgG ELISA試劑盒
human transfusion transmitted virus,TTV ELISA試劑盒
Human Hepatitis D virus IgM,HDV IgM ELISA試劑盒
human hepatitis E virus IgG,HEV-IgG ELISA試劑盒
Human Hepatitis D virus IgM,HDV IgM ELISA試劑盒
human Hepatitis D virus IgG,HDV IgG ELISA試劑盒
Human Hepatitis C virus IgM,HCV-IgM ELISA試劑盒
Human Hepatitis C virus IgG,HCV-IgG ELISA試劑盒
Human glucoprotein 130,gp130 ELISA試劑盒
Human corticotropin-releasing factor,CRF
Human Desmopressin/1-desamino-8-D-arginine vasopressin,dDAVP ELISA試劑盒
Human muscarinic acetylcholine receptor M3,M-AChR M3 ELISA試劑盒
Human Testoterone recepter,TR ELISA試劑盒
Human muscarinic acetylcholine receptor,M-AChR ELISA試劑盒
human glycoprotein 49A,Gp49a ELISA試劑盒
human inhibin beta-B,Inhbb ELISA試劑盒
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Human calmodulin,CAM ELISA試劑盒
Human true insulin,TI ELISA試劑盒
Human Noradrenaline Bitartas,NE-B ELISA試劑盒
Human prostacycline,PGI ELISA試劑盒
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QY-R95195 Rabbit Interleukin 8,IL-8 ELISA Kit 兔子白介素8(IL-8CXCL8)檢測試劑盒 ELISA. 96T48T
QY-R95196 Rabbit Interleukin 6,IL-6 ELISA Kit 兔子白介素6(IL-6)檢測試劑盒 ELISA. 96T48T
QY-R95197 Rabbit Interleukin 4,IL-4 ELISA Kit 兔子白介素4(IL-4)檢測試劑盒 ELISA. 96T48T
QY-R95198 Rabbit Interleukin 3,IL-3 ELISA Kit 兔子白介素3(IL-3)檢測試劑盒 ELISA. 96T48T
QY-R95200 Rabbit Interleukin 1β,IL-1β ELISA Kit 兔子白介素1β(IL-1β)檢測試劑盒 ELISA. 96T48T
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QY-R95204 Rabbit Soluble protein-100B,S-100B ELISA Kit 兔子S100B蛋白(S-100B)檢測試劑盒 ELISA. 96T48T
QY-R95205 Rabbit Soluble protein-100,S-100 ELISA Kit 兔子S100蛋白(S-100)檢測試劑盒 ELISA. 96T48T
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QY-R95210 Rabbit Collagen Type Ⅰ,Col Ⅰ ELISA Kit 兔子Ⅰ型膠原(ColⅠ)檢測試劑盒 ELISA. 96T48T
QY-R95211 Rabbit Collagen Type Ⅱ,Col Ⅱ ELISA Kit 兔子Ⅱ型膠原(ColⅡ)檢測試劑盒 ELISA. 96T48T
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QY-R95220 Rabbit Progesterone,PROG ELISA Kit 兔子孕激素孕酮(PROG)檢測試劑盒 ELISA. 96T48T
QY-R95221 Rabbit Collagenase Type II ELISA Kit 兔子膠原酶II(CollagenaseII)檢測試劑盒 ELISA. 96T48T
QY-R95222 Rabbit Collagenase I ELISA Kit 兔子膠原酶I(CollagenaseI)檢測試劑盒 ELISA. 96T48T
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魚
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QY-F96002 Salmon calcitonin,SCT ELISA Kit 鮭魚降鈣素(SCT)檢測試劑盒 ELISA. 96T48T
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