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當前位置:齊一生物科技(上海)有限公司>>ELISA試劑盒>>大鼠ELISA試劑盒>> 大鼠Ⅰ型膠原交聯(lián)羧基末端肽(ⅠCTP)檢測試劑盒
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更新時間:2015-10-30 15:57:08瀏覽次數(shù):200次
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大鼠Ⅰ型膠原交聯(lián)羧基末端肽(ⅠCTP)檢測試劑盒齊一生物科技(上海)有限公司新品*歡迎您訪問我們的如有什么問題請給我們或者留言741653262,告訴我您的需要,并留下您寶貴的意見和建議
大鼠Ⅰ型膠原交聯(lián)羧基末端肽(ⅠCTP)檢測試劑盒注意事項:
1. 試劑盒從冷藏環(huán)境中取出應在室溫平衡15-30分鐘后方可使用,酶標包被板開封后如未用完,板條應裝入密封袋中保存。
2. 濃洗滌液可能會有結(jié)晶析出,稀釋時可在水浴中加溫助溶,洗滌時不影響結(jié)果。
3. 各步加樣均應使用加樣器,并經(jīng)常校對其準確性,以避免試驗誤差。一次加樣時間控制在5分鐘內(nèi),如標本數(shù)量多,使用排槍加樣。
4. 請每次測定的同時做標準曲線,做復孔。如標本中待測物質(zhì)含量過高(樣本OD值大于標準品孔*孔的OD值),請先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測定,計算時請zui后乘以總稀釋倍數(shù)(×n×5)。
5. 封板膜只限一次性使用,以避免交叉污染。
6. 底物請避光保存。
7. 嚴格按照說明書的操作進行,試驗結(jié)果判定必須以酶標儀讀數(shù)為準.
8. 所有樣品,洗滌液和各種廢棄物都應按傳染物處理。
9. 本試劑不同批號組分不得混用。
10. 如與英文說明書有異,以英文說明書為準。
計算:
以標準物的濃度為橫坐標,OD值為縱坐標,在坐標紙上繪出標準曲線,根據(jù)樣品的OD,值由標準曲線查出相應的濃度;再乘以稀釋倍數(shù);或用標準物的濃度與OD值計算出標準曲線的直線回歸方程式,將樣品的OD值代入方程式,計算出樣品濃度,再乘以稀釋倍數(shù),即為樣品的實際濃度。
試劑盒性能:
1.樣品線性回歸與預期濃度相關(guān)系數(shù)R值為0.95以上。
2.批內(nèi)與批見應分別小于9%和11%
保存條件及有效期:
1.試劑盒保存;2-8℃。
2.有效期:6個月 齊一生物科技有限公司主要經(jīng)營進口原裝Elisa試劑盒,種類齊全,價格實惠,購買我公司產(chǎn)品的新老客戶,可享免費代測實驗服務,可檢測人源,小鼠,大鼠,豬,狗,猴,兔,羊,雞,牛,鴨子,魚,植物等各種樣本。全程提供。如有什么問題請給我們或者留言,告訴我您的需要,并留下您寶貴的意見和建議
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