當前位置:齊一生物科技(上海)有限公司>>ELISA試劑盒>>人ELISA試劑盒>> 齊一生物促胰液素(secretin)檢測試劑盒
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齊一生物促胰液素(secretin)檢測試劑盒齊一生物科技(上海)有限公司以真誠、主動、周到、規范、熱情的服務很快得到廣大客戶的*好評。公司秉承“適應多樣化的需求,提供專業化的服務"的經營理念,為客戶提供*的服務。歡迎社會各界朋友前來洽談業務,共創輝煌
齊一生物促胰液素(secretin)檢測試劑盒標本的采集及保存:
1.血清:室溫血液自然凝固10-20分鐘后,離心20分鐘左右(2000-3000轉/分)。仔細收集上清。保存 過程 中 如有沉淀形成,應再次離心。
2.血漿:應根據標本的要求選擇EDTA、檸檬酸鈉或肝素作為抗凝劑,混合10-20分鐘后,離心20分鐘左右(2000-3000轉/分)。仔細收集上清。保存過程中如有沉淀形成,應再次離心。
3.尿液:用無菌管收集。離心20分鐘左右(2000-3000轉/分)。仔細收集上清。保存過程中如有沉淀形成,應再次離心。胸腹水、腦脊液參照此實行。
4.細胞培養上清:檢測分泌性的成份時,用無菌管收集。離心20分鐘左右(2000-3000轉/ 分)。仔細收集上清。 檢測細胞內的成份時,用PBS(PH7.2-7.4)稀釋細胞懸液,細胞濃度達到100萬/ml左右。通過反復凍融,以 使細胞破壞并 放出細胞內成份。離心20分鐘左右(2000-3000轉/分)。仔細收集上清。保存過程中如有沉淀形成,應再次離心。
5.組織標本:切割標本后,稱取重量。加入一定量的PBS,PH7.4。用液氮迅速冷凍保存備用。標本融化后仍然保持2-8℃的溫度。加入一定量的PBS(PH7.4),用手工或勻漿器將標本勻漿充分。離心20分鐘左右(2000-3000轉/分)。仔細收集上清。分裝后一份待檢測,其余冷凍備用。
標本要求:
1.標本采集后盡早進行提取,提取按相關文獻進行,提取后應盡快進行實驗。若不能馬上進行試驗,可將標本放于-20℃保存,但應避免反復凍融
2.不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
試劑盒組成:
48孔配制:
1. 說明書: 1份
2. 封板膜: 2片(48)
3. 密封袋: 1個
4 酶標包被板: 1×48 (2-8℃保存)
5. 標準品:0.5ml×1瓶(2-8℃保存)
6. 標準品稀釋液: 1.5ml×1瓶(2-8℃保存)
7. 酶標試劑: 3 ml×1瓶(2-8℃保存)
8. 樣品稀釋液: 3 ml×1瓶(2-8℃保存)
9. 顯色劑A液: 3 ml×1瓶(2-8℃保存)
10 .顯色劑B液: 3 ml×1瓶(2-8℃保存)
11 .終止液:3ml×1瓶(2-8℃保存)
12. 濃縮洗滌液:(20ml×20倍)×1瓶(2-8℃保存)
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T2R14 Polyclonal Antibody Human WB IF ELISA
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29988 Recombinant Protein G, Biotinylated 0.5 mg
29989 Protein A, Biotinylated 1 mg
29994 Pierce Avidin, Horseradish Peroxidase Conjugated 5 mg
29997 Recombinant Protein L, Biotinylated 0.5 mg
30030 Dulbecco's Modified Eagle's Limiting Medium (DMEM-LM) 500 mL
31000 NeutrAvidin Protein 10 mg
31001 NeutrAvidin Protein, Horseradish Peroxidase Conjμgated 2 mg
31002 NeutrAvidin Protein, Alkaline Phosphatase Conjμgated 2 mg
31006 NeutrAvidin Protein, Fluorescein Conjugated 5 MG
31007 Maleimide-Activated NeutrAvidin Protein 5 mg
31030 High Sensitivity NeutrAvidin-HRP 0.5 mL
31032 High Sensitivity NeutrAvidin-HRP 5 mL
31050 NeutrAvidin Protein 100 mg
31055 NeutrAvidin Protein 1000 mg
31104 Pierce(R) Rabbit Anti-Chicken IgY (H+L) 2.0 MG
31105 Pierce(R) Rabbit Anti-Goat IgG (H+L), 2 MG 2 MG
31107 Pierce(R) Mouse Anti-Goat IgG (H+L) (min x HnMsRb Sr Prot) 1.5 MG
31109 Pierce(R) F(ab')2 Fragment Rabbit Anti-Goat IgG (H+L) (mi 0.5 MG
31111 Immunodiffusion Plates, Agarose Gelling Agent, Multiple Pattern 6/PKG
31115 Pierce(R) Goat Anti-Hamster IgG (H+L) 1.5 MG
31118 Pierce(R) Goat Anti-Human IgG (Gamma) 0.5 MG
31119 Pierce(R) Goat Anti-Human IgG (H+L), (min x BvHsMs Sr 1.5 MG
31122 Pierce(R) Goat Anti-Human IgG {F(ab')2} 2.0 MG
31124 Pierce(R) Goat Anti-Human IgM (u) 0.5 MG
31125 ImmunoPure Goat Anti-Human IgG (Fc, (min x BvHsMs Sr Prot 1 MG
31128 Pierce(R) Goat Anti-Human IgA + IgG + IgM (H+L) 2.0 MG
31129 Pierce(R) Goat Anti-Human Kappa Chain 0.5 MG
31130 Pierce(R) Goat Anti-Human IgG (H+L), 2 mg 2 MG
31131 Pierce(R) Goat Anti-Human Lambda Chain 0.5 MG
31132 Pierce(R) Goat Anti-Human IgG F(ab')2, (min X Bv Hs Ms Sr P 1.5 MG
31133 Pierce(R) Rabbit Anti-Goat IgG (Fc) 2.0 MG
31135 Pierce(R) Mouse Anti-Human IgG (H+L), (min x Ms Sr Prot) 2.0 MG
31136 Pierce(R) GOAT ANTI-HUMAN IgM Fc5u, 2 MG 2 MG
31137 Pierce(R) Mouse Anti-Human IgG (H+L), (min x BvHsMs Sr 1.5 MG
31140 Pierce(R) Goat Anti-Human Serum IgAa, 2 MG 2 MG
31142 Pierce(R) Rabbit Anti-Human IgG Fc, 2 MG 2 MG
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