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當前位置:齊一生物科技(上海)有限公司>>ELISA試劑盒>>人ELISA試劑盒>> 齊一生物組織型纖維溶酶激活物(t-PA)檢測試劑盒

齊一生物組織型纖維溶酶激活物(t-PA)檢測試劑盒

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更新時間:2015-10-29 12:03:16瀏覽次數:151次

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齊一生物組織型纖維溶酶激活物(t-PA)檢測試劑盒價格:全國:或咨詢在線客服,或者以郵件形式發到我司741653262,齊一生物科技(上海)有限公司提供大量品牌進口原裝,分裝,以及穩定性強的國產ELISA試劑盒,性價比*

齊一生物組織型纖維溶酶激活物(t-PA)檢測試劑盒價格:全國:或咨詢在線客服,或者以郵件形式發到我司741653262,齊一生物科技(上海)有限公司提供大量品牌進口原裝,分裝,以及穩定性強的國產ELISA試劑盒,性價比*

齊一生物組織型纖維溶酶激活物(t-PA)檢測試劑盒有效期:6個月
預期應用:ELISA法定量測定血清、血漿、細胞培養上清或其它相關生物液體中的含量.
說明
1.試劑盒保存:-20℃(較長時間不用時);2-8℃(頻繁使用時).
2.濃洗滌液低溫保存會有鹽析出,稀釋時可在水浴中加溫助溶.
3.中、英文說明書可能會有不*之處,請以英文說明書為準.
4.剛開啟的酶聯板孔中可能會含有少許水樣物質,此為正常現象,不會對實驗結果造成任何影響.
實驗原理
用純化的抗體包被微孔板,制成固相載體,往包被抗該指標抗體的微孔中依次加入標本或標準品、化的抗該指標抗體、HRP標記的親和素,經過*洗滌后用底物TMB顯色.TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成zui終的黃色.顏色的深淺和樣品中的該指標呈正相關.用酶標儀在450nm波長下測定吸光度(OD值),計算樣品濃度.
試劑盒組成及試劑配制
1.         酶聯板(Assay plate ):一塊(96孔).
2.         標準品(Standard):2瓶(凍干品).
3.         樣品稀釋液(Sample Diluent):1×20ml/瓶.
4.         標記抗體稀釋液(Biotin-antibody Diluent):1×10ml/瓶.
5.         辣根過氧化物酶標記親和素稀釋液 (HRP-avidin Diluent):1×10ml/瓶.
6.         標記抗體(Biotin-antibody):1×120μl/瓶(1:100)
7.         辣根過氧化物酶標記親和素(HRP-avidin):1×120μl/瓶(1:100)
8.         底物溶液(TMB Substrate):1×10ml/瓶.
9.         濃洗滌液(Wash Buffer):1×20ml/瓶,使用時每瓶用蒸餾水稀釋25倍.
10.     終止液(Stop Solution):1×10ml/瓶(2N H2SO4).
19541    QIAvac Luer Adapter Set    For processing 1-24 QIAGEN spin columns on QIAvac 6S: 6 adapters with 4 luer connectors each, 24 plugs
19577    48-Well Blocks (24)    48-well blocks with 5 ml wells, 24 per case
9017734    Plasticware kit, IQ/OQ, EZ1
9019885    Cover, main, label Biocontrol, RG3
9019907    Switch - Vacuum VPT, PyroQ24VW
9019012    Motor Y, EZ1xl
9019025    Worktable, side wall, left, EZ1xl
9019729    Board, motor control, Z, V2, QX
9019745    EZ1 Adv. XL DNA Dried Blood Card
870501    ALK RGQ RT-PCR Kit (24)
36912    d12-Tube Magnet    Magnet for separating magnetic beads in 12 x 1.5 ml or 2 ml tubes
51185    QIAamp DNA Blood Midi Kit (100)    For 100 DNA midipreps: 100 QIAamp Midi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (15 ml)
68163    DNeasy Plant Maxi Kit (24)    24 DNeasy Maxi Spin Columns, 24 QIAshredder Maxi Spin Columns, RNase A, Buffers, Collection Tubes (50 ml)
79216    Buffer RLT    220 ml RNeasy Lysis Buffer
120007    Collection Microtubes and Caps    Nonsterile polypropylene tubes (1.2 ml), 2304 in packs of 96, and nonsterile polypropylene caps for Collection Microtubes
201207    Taq DNA Polymerase (5000)    "20 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity and minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye and a red dye, that facilitate estimation of DNA migration distance and optimization of agarose gel run time."
132559    NeXtal Stock Magnesium acetate (50)    1M Magnesium acetate tetrahydrate - 50mL (4 x 12,5 ml tube)
9019585    Slideout electrical tray, QGY
9019599    Housing, filter seal, CX1
9019602    Home flag, Y-axis, Cx8
9019649    Fuse kit (6.3Ax10, 5Ax5), PyroQ96
970012    PyroMark Q24 CpG p16 (4 x 24)    For quantification of methylation level in  region +148 to +174 in exon 1 of the gene.
972824    PyroMark Gold Q96 CDT Reagents (6 x 96)    For performing pyrosequencing reaction on the Q96 MD in combination with the capillary dispensing tips (CDT)
9019668    PCB - Valve, PyroQ24
950911    Capture Plate, DX (5)
981313    Rotor-Disc 100 (300)
1070498    Buffer TCL, 2x (8.5 ml),G
206624    Type-it CNV SYBR Green PCR Core Kit(400)
19055    Buffer QC    1000 ml Wash Buffer
13362    Blood & Cell Culture DNA Maxi Kit    10 QIAGEN Genomic-tip 500/G, QIAGEN Protease, Buffers
19064    Buffer N3    500 ml Neutralization Buffer
19081    Buffer AW1    242 ml Wash Buffer (1) Concentrate for 1000 spin, 250 midi, or 100 maxi preps
19530    Vacuum Regulator    For use with QIAvac manifolds
28304    QIAquick Nucleotide Removal Kit (50)    50 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)
9012730    Cable, flat band, X, 2300 mm
9013173    Pump, probe flush, BR8000
4510033    artus Enterovirus LC RT-PCR Kit (24) PRE
130718    NeXtal Tubes PACT Suite
19088    Reagent DX
218411    Human miScript Primer Assay Set VXX.X
9019899    Cable - Ground, PyroQ96ID
9019003    Hinge, for door, EZ1xl
9019004    Grid, UV lamp, EZ1xl
9019021    Damper, door, EZ1xl
9019759    Power supply, main, 24V, V2, QX
950154    Wash Reagent (DXW) (650 ml)
9019537    Plate Stacker A/B RD, RCS
9021031    Frame, transfer module, Qsym
337042    qBiomarker Assay PreAMP Mix
51161    QIAamp 96 DNA Blood Kit (4)    For 4 x 96 DNA preps: 4 QIAamp 96 Plates, QIAGEN Protease, Reagents, Buffers, Lysis Blocks, Tape Pads, Collection Vessels
75162    RNeasy Maxi Kit (12)    12 RNeasy Maxi Spin Columns, Collection Tubes (50 ml), RNase-free Reagents and Buffers
120016    QIAquick 96 Plates (24)    Individual QIAquick 96 plates and handbook. No buffers or reagents.
203443    HotStarTaq Master Mix Kit (250)    "3 x 0.85 ml HotStarTaq Master Mix* containing 250 units HotStarTaq DNA Polymerase total, 2 x 1.7 ml distilled water

- premixed solution for high PCR specificity
- HotStarTaq Master Mix, HotStarTaq DNA Polymerase, several kit sizes from 250 u up to 2500 u available"
301108    PolyFect Transfection Reagent (100 ml)    For 2500-6500 transfections in 60 mm dishes or 5000-10,000 transfections in 6-well plates.
231222    QIAGEN PCR Cloning plus Kit (10)    For 10 reactions: 2x Ligation Master Mix (50 ul), pDrive Cloning Vector (0.5 ug), distilled water (1.7 ml), QIAGEN EZ Competent Cells (10 tubes, 50 ul each), SOC medium (2 x 1.9 ml)
9015638    AC fuse, 115 V, 250 8A, M48
132568    NeXtal Stock Methoxy-PEG 5000 (50)    30% w/v Methoxypolyethylene glycol 5000 - 50mL (4 x 12,5 ml tube)
9019577    Opto switch, Z-axis, 1 ch. , Cx2
9019594    Belt, Z-axis gearbox (black), 8 ch., Cx8
9019068    PyroMark CpG SW 1.0 (5)    "Software for PyroMark Q96 ID and PyroMark Q96 MD for analysis and quantification of CpG methylation in epigenetic studies. 
Five additional software licenses for PyroMark CpG SW 1.0"
9019086    PyroMark Q96 MD Software (5)    "For use with PyroMark Q96 MD instrument.Software for setting up a run and analyzing mutations.

5 licenses"
9019658    O rings - VPT Fast connector, PyroVW
972012    PyroMark Q96 CpG p16 (96)    For quantification of methylation level in p16
979003    PyroMark Q96 Sample Prep Tool Cover (10)    Consumables for use with magnetic tool for isolation of single stranded DNA.
979008    PyroMark Wash Buffer (conc., 200 ml)    For washing and neutralizing the immobilized DNA.
950140    Binding Additive (DX) (4.0 g)
9019803    Upgrade Package 1, RCS
130803    NeXtal CubicPhase 1w Mo μplate (10)
9243044    Set, brackets, transparent panel, Qsym
283615    virotype PEDV/TGEV RT-PCR Rgt (96)
52906    QIAamp Viral RNA Mini Kit (250)    For 250 RNA preps: 250 QIAamp Mini Spin Columns, Carrier RNA, Collection Tubes (2 ml), RNase-free Buffers
63183    DyeEx 96 Kit (24)    24 DyeEx 96 Plates; 4 Collection Plates, 48-Well
69582    DNeasy 96 Blood & Tissue Kit (12)    For 12 x 96 DNA minipreps: 12 DNeasy 96 Plates, QIAGEN Proteinase K, Buffers, Square-Well Blocks, AirPore Tape Sheets, Collection Microtubes (1.2 ml), Caps, 96-well Plate Registers
70042    Oligotex mRNA Midi Kit    For 12 mRNA midipreps: 700 ul Oligotex Suspension, Small Spin Columns, Collection Tubes (1.5 ml), RNase-free Reagents and Buffers
79523    Small Spin Columns    24 RNase-free spin columns for Oligotex spin procedures
301109    PolyFect Transfection Reagent (10x100ml)
9239361    PCB-micro-fuse T 6.3A, 250V, EZ1
132632    NeXtal Stock Potassium formate (50)    10M Potassium formate - 50mL (4 x 12,5 ml tube)
9019610    Cover, Pipettor, 1 ch. (white), CAS
9019624    PCB, lge breakout, 12 core to rev 3, Cx2
9019067    PyroMark CpG SW 1.0    Software for PyroMark Q96 ID and PyroMark Q96 MD for analysis and quantification of CpG methylation in epigenetic studies.
9019087    PyroMark IdentiFire SW 1.0    Software for sequence-based species identification by comparing and matching Pyrosequencing data to a local database.
972052    PyroMark Q96 CpG Prader Willi (96)    For quantification of methylation level in  Prader-Willi and Angelman Syndromes
979002    PyroMark Q96 Plate Low (100)    For sample analysis of DNA template on ID.
979004    PyroMark Q96 Cartridge (3)    For delivery of nucleotides and reagents for use in PyroMark Q96 ID.
9001513    PyroMark Q24 MDx    For methylation and mutation analysis using Pyrosequencing technology in 24 well CE-marked.
990610    Filter-Tips, OnCor C, 200 μl (960)
9017842    Tool, gauge, 30 mm, calibration, QIAC
9020806    Rotor Timing Calibration Dye, RGQ
331455    miScript Microfluidics PreAMP Kit (96)
381566    Investigator ESSplex SE GO! Kit (200)
9022654    Transfer Carriage QIAplate, QXT
16191    QIAwell 96 Ultra Plasmid Kit (4)    For 4 x 96 ultrapure plasmid minipreps, 4 each: QIAfilter 96, QIAwell 96, and QIAprep 96 Plates; Reagents, Buffers, Collection Microtubes (1.2 ml), Caps
19046    Plasmid Buffer Set    Buffers P1, P2, P3, QBT, QC, QF, RNase A; for 100 plasmid mini-, 25 midi-, or 10 maxipreps
19201    Collection Tubes (2-ml)    1000 Collection Tubes (2 ml)
9015862    EZ1 DNA PRFN Card    Pre-programmed card for BioRobot EZ1 paraffin section protocols
67163    MagAttract 96 DNA Plant Core Kit (24)    MagAttract Suspension A and buffers for 24 x 96 preps
1030483    Elution Microtubes CL, PAX, FLU
9232687    Hood f. Tip Disposal Station 2.01
11AGAH0500     Agarose, Mol. Bio. Grade, low EEO     瓊脂糖,分子生物學級別    500 g
04800655    Acrylamide/Bis Premix 19:1    丙烯酰胺/甲叉雙丙烯酰胺 19:1    30 g
04800656    Acrylamide/Bis Premix  19:1    丙烯酰胺/甲叉雙丙烯酰胺 19:1    200 g
04800657    Acrylamide/Bis Premix  29:1    丙烯酰胺/甲叉雙丙烯酰胺 29:1    30 g
04800658    Acrylamide/Bis Premix  29:1    丙烯酰胺/甲叉雙丙烯酰胺 29:1    200 g
04800659    Acrylamide/Bis Premix  37.5:1    丙烯酰胺/甲叉雙丙烯酰胺 37.5:1    30 g
04800660    Acrylamide/Bis Premix 37.5:1    丙烯酰胺/甲叉雙丙烯酰胺 37.5:1    200 g
    Acrylamide    丙烯酰胺    100 g
    Acrylamide    丙烯酰胺    500 g
    Acrylamide    丙烯酰胺    1 kg
    Acrylamide Molecular Biology Grade     丙烯酰胺 分子生物學級別    25 g
    Acrylamide Molecular Biology Grade     丙烯酰胺 分子生物學級別    100 g
    Acrylamide Molecular Biology Grade     丙烯酰胺 分子生物學級別    500 g
    Acrylamide Molecular Biology Grade     丙烯酰胺 分子生物學級別    1 kg
04814340    Acrylamide Electrophoresis Grade    丙烯酰胺 電泳級別    100 g
04814343    Acrylamide Electrophoresis Grade    丙烯酰胺 電泳級別    250 g
04814346    Acrylamide Electrophoresis Grade    丙烯酰胺 電泳級別    500 g
04814349    Acrylamide Electrophoresis Grade    丙烯酰胺 電泳級別    1 kg
04814320    Acrylamide, Ultra Pure    丙烯酰胺 超純    100 g
04814323    Acrylamide, Ultra Pure    丙烯酰胺 超純    250 g
04814326    Acrylamide, Ultra Pure    丙烯酰胺 超純    500 g
04814329    Acrylamide, Ultra Pure    丙烯酰胺 超純    1 kg
 11BIAC1902      Acrylamide-Bis-Acryl 19:1     丙烯酰胺-甲叉雙丙烯酰胺 19:1     1 L 
 11BIAC2902      Acrylamide-Bis-Acryl 19:1     丙烯酰胺-甲叉雙丙烯酰胺 29:1     1 L 
 11BIAC3752      Acrylamide-Bis-Acryl  37,5:1     丙烯酰胺-甲叉雙丙烯酰胺 37.5:1     1 L 
 04800802      LIQUI-GEL? 19:1 (40% Acrylamide Bis - Acrylamide Solution)      LIQUI-GEL? 19:1(40%丙烯酰胺-甲叉雙丙烯酰胺溶液)      500 ml 
 04800803      LIQUI-GEL? 29:1 (40% Acrylamide Bis - Acrylamide Solution)      LIQUI-GEL? 29:1(40%丙烯酰胺-甲叉雙丙烯酰胺溶液)      500 ml 
04800804     LIQUI-GEL? 37.5:1 (40% Acrylamide Bis - Acrylamide Solution)      LIQUI-GEL? 37.5:1(40%丙烯酰胺-甲叉雙丙烯酰胺溶液)      500 ml 
緩沖液            
       DTA Disodium Salt, Dihydrate, Molecular Biology reagen     EDTA二鈉鹽     500 g 
 11EDTA05M1      EDTA EASY SOL     EDTA二鈉鹽溶液     100 ml 
 112350200      ETBR Green Bag Disposal Kit     溴化乙錠去除試劑盒     50 bags 
 04802511      Ethidium Bromide, Solution (10mg/ml)     溴化乙錠溶液     10 ml 
 11TAE50X01      TAE 50X Buffer     TAE 50X 緩沖液     1 L 
 11TBE10X02      TBE 10X Buffer     TBE 10X 緩沖液      1 L 
 04816116      Tris HCl, Ultra Pure     TRIS HCL,超純     100 g 
       Tris, Molecular Biology Reagent     TRIS,分子生物學級別     1 kg 
培養基            
    Casein, Vitamin Tested 30 Mesh    酪蛋白    1 lb
    Casein, Vitamin Tested 30 Mesh    酪蛋白    5 lb
    Casein, Vitamin Tested 30 Mesh    酪蛋白    25 lb
    Casein, Vitamin Tested 30 Mesh    酪蛋白    100 lb
    Agar (Bacteriological Grade)    瓊脂(細菌學級別)    100 g
    Agar (Bacteriological Grade)    瓊脂(細菌學級別)    250 g
    Agar (Bacteriological Grade)    瓊脂(細菌學級別)    500 g
    Casein Hydrolysate    酪蛋白水解物    1 lb
    Casein Hydrolysate    酪蛋白水解物    5 lb
    Casein Hydrolysate    酪蛋白水解物    25 lb
    Zein    玉米醇蛋白    100 g
    Zein    玉米醇蛋白    500 g
    Zein    玉米醇蛋白    1 kg
    Yeast Hydrolysate Enzymatic    酵母水解酶    1 lb
    Yeast Hydrolysate Enzymatic    酵母水解酶    5 lb
    Yeast Hydrolysate Enzymatic    酵母水解酶    25 lb
    Yeast Hydrolysate Enzymatic    酵母水解酶    100 lb
    Yeast Extract Powder    酵母粉    100 g
    Yeast Extract Powder    酵母粉    500 g
    Yeast Extract Powder    酵母粉    1 kg
    Agar, NF Grade    瓊脂,NF級別    100 g
    Agar, NF Grade    瓊脂,NF級別    250 g
    Agar, NF Grade    瓊脂,NF級別    500 g
    Agar, NF Grade    瓊脂,NF級別    1 kg
115100200    Plant Surface Sterilization Kit    植物表面滅菌試劑盒    50 preps
115000550    ROLL & GROW? PLATING BEADS    涂布珠    2 tubes
115000009    Velvet Pad, 9 Inch Square (Pack)(Pouches)    平板    150 mm
115000007    Velvet Pad, 6 Inch Square (Pack)    平板    100 mm
115000004    Replica Plating Apparatus (1 Unit)    Replica Plating Apparatus平板    150 mm
115000001    Replica Plating Apparatus (1 Unit)    Replica Plating Apparatus 平板    100 mm
114842185    SDA Medium-Ade-His-Leu-Trp (Case)(Pouches)    SDA Medium-Ade-His-Leu-Trp 合成篩選培養基    100x0.5 L
114842165    SDA Medium-Ade-His-Leu-Trp (Pack)(Pouches)    SDA Medium-Ade-His-Leu-Trp  合成篩選培養基    10x0.5 L
114842075    SD Medium-Ade-His-Leu-Trp (Pack)(Pouches)    SD Medium-Ade-His-Leu-Trp 合成篩選培養基    10x1.0 L
114842065    SD Medium-Ade-His-Leu-Trp (Pack)(Pouches)    SD Medium-Ade-His-Leu-Trp  合成篩選培養基    10x0.5 L
114840185    SDA Medium-His-Leu-Trp-Ura (Case)(Pouches)    SDA Medium-His-Leu-Trp-Ura  合成篩選培養基    100x0.5 L
114840175    SDA Medium-His-Leu-Trp-Ura (Pack)(Pouches)    SDA Medium-His-Leu-Trp-Ura  合成篩選培養基    10x1.0 L
114840165    SDA Medium-His-Leu-Trp-Ura (Pack)(Pouches)    SDA Medium-His-Leu-Trp-Ura 合成篩選培養基    10x0.5 L
114840095    SDMedium-His-Leu-Trp-Ura (Case)(Pouches)    SDMedium-His-Leu-Trp-Ura  合成篩選培養基    100x1.0 L
114840075    SD Medium-His-Leu-Trp-Ura (Pack)(Pouches)    SD Medium-His-Leu-Trp-Ura  合成篩選培養基    10x1.0 L
114840065    SD Medium-His-Leu-Trp-Ura Pk    SD Medium-His-Leu-Trp-Ura Pk 合成篩選培養基    10x0.5 L
114833175    SDA Medium-Leu-Trp-Ura (Powder)    SDA Medium-Leu-Trp-Ura  合成篩選培養基    10x1.0 L
114830175    SDA Medium-His-Leu-Trp (Pack)(Pouches)    SDA Medium-His-Leu-Trp 合成篩選培養基    10x1.0 L
114830165    SDA Medium-His-Leu-Trp (Pack)(Pouches)    SDA Medium-His-Leu-Trp  合成篩選培養基    10x0.5 L
114830095    SD Medium-His-Leu-Trp (Case)(Pouches)    SD Medium-His-Leu-Trp  合成篩選培養基    100x1.0 L
114830075    SD Medium-His-Leu-Trp (Pack)(Pouches)    SD Medium-His-Leu-Trp 合成篩選培養基    10x1.0 L

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