齊一生物糖皮質激素（GC）檢測試劑盒齊一生物科技（上海）有限公司產品已被廣泛應用于化學、化工、生命科學的基礎研究和開發應用、制藥、疾病診斷與控制、人口與健康、生物技術等諸多領域.客戶遍布國內各大學、研究所、醫院、衛生防疫、商品檢驗檢疫、制藥公司、生物技術公司和食品工業等單位.

齊一生物糖皮質激素（GC）檢測試劑盒ELISA試劑盒操作步驟
ELISA試劑盒使用前,將所有試劑充分混勻.不要使液體產生大量的泡沫,以免加樣時加入大量的氣泡,產生加樣上的誤差.
根據待測樣品數量加上標準品的數量決定所需的板條數.每個標準品和空白孔建議做復孔.每個樣品根據自己的數量來定,能使用復孔的盡量做復孔.標本用標本稀釋液1：1稀釋后加入50ul于反應孔內.
加入稀釋好后的標準品50ul于反應孔、加入待測樣品50ul于反應孔內.立即加入50ul的標記的抗體.蓋上膜板,輕輕振蕩混勻,37℃溫育1小時.
甩去孔內液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復此操作3次.如果用洗板機洗滌,洗滌次數增加一次.
每孔加入80ul的親和鏈酶素-HRP,輕輕振蕩混勻,37℃溫育30分鐘.
甩去孔內液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復此操作3次.如果用洗板機洗滌,洗滌次數增加一次.
每孔加入底物A、B各50ul,輕輕振蕩混勻,37℃溫育10分鐘.避免光照.
取出酶標板,迅速加入50ul終止液,加入終止液后應立即測定結果.
在450nm波長處測定各孔的OD值.
ELISA操作步驟
human schistosoma ELISA試劑盒
Human Rotavirus antigen,RV Ag ELISA試劑盒
Human Legionella pneumophila antigen,LP Ag ELISA試劑盒
human colicin ELISA試劑盒
Human Adenovirus Antigen,ADV-Ag ELISA試劑盒
Human Trichinella antibody ELISA試劑盒
Human Tetanus Antibody ELISA試劑盒
Human Lebtospira IgM ELISA試劑盒
Human Leptospira IgG,Lep IgG ELISA試劑盒
Human Amebiasis ELISA試劑盒
Human Diphtheria antibody ELISA試劑盒
Human Cysticercosis antybody,CYT Ab ELISA試劑盒
Human Brucella Antibody IgG,Brucella Ab IgG ELISA試劑盒
Human Varicella zoster virus IgM,VZV IgM ELISA試劑盒
Human Varicella zoster virus IgG,VZV-IgG ELISA試劑盒
Human Legionella antibody IgA,LP Ab-IgA ELISA試劑盒
Human Legionella antibody IgM,LP Ab-IgM ELISA試劑盒
Human Legionella antibody IgG,LP Ab-IgG ELISA試劑盒
Human paramyxovirus parotitis IgM ELISA試劑盒
Human paramyxovirus parotitis IgG ELISA試劑盒
Human epstein-barr virus IgM,EBv IgM ELISA試劑盒
Human epstein-barr virus IgA,EBv IgA ELISA試劑盒
Human epstein-barr virus IgG,EBv IgG ELISA試劑盒
Human Leishimaria antibody ELISA試劑盒
Human dengue fever antibody,DF ELISA試劑盒
Human Influenza viruses IgG,FLU ELISA試劑盒
Human Adenovirus IgM,ADV-IgM ELISA試劑盒
Human Adenovirus IgG,ADV-IgG ELISA試劑盒 
Human Rotavirus,RV IgM ELISA試劑盒
human EchoVirus IgM,ECHO IgM ELISA試劑盒
Human anti-respiratory syncytial virus antibody,RSV ELISA試劑盒
Human measlesvirus IgM,MV IgM ELISA試劑盒
Human Coxsackie virus IgM,Cox V-IgM ELISA試劑盒
Human Coxsackie virus IgG,Cox V-IgG ELISA試劑盒
Human tuberculosis antibody IgG,TB-Ab IgG ELISA試劑盒
Human cicatricial Pemphigoid,CP ELISA試劑盒
human hepatitis B virus,HBV preS2Ab ELISA試劑盒
human hepatitis B virus,HBV preS2Ag ELISA試劑盒
Human Hepatitis G virus IgG,HGV-IgG ELISA試劑盒
human transfusion transmitted virus,TTV ELISA試劑盒
Human Hepatitis D virus IgM,HDV IgM ELISA試劑盒
human hepatitis E virus IgG,HEV-IgG ELISA試劑盒
Human Hepatitis D virus IgM,HDV IgM ELISA試劑盒
human Hepatitis D virus IgG,HDV IgG ELISA試劑盒
Human Hepatitis C virus IgM,HCV-IgM ELISA試劑盒
Human Hepatitis C virus IgG,HCV-IgG ELISA試劑盒
Human glucoprotein 130,gp130 ELISA試劑盒 
Human corticotropin-releasing factor,CRF 
Human Desmopressin/1-desamino-8-D-arginine vasopressin,dDAVP ELISA試劑盒
Human muscarinic acetylcholine receptor M3,M-AChR M3 ELISA試劑盒 
Human Testoterone recepter,TR ELISA試劑盒
Human muscarinic acetylcholine receptor,M-AChR ELISA試劑盒
human glycoprotein 49A,Gp49a ELISA試劑盒
human inhibin beta-B,Inhbb ELISA試劑盒
Human Orexin receptor,OXR ELISA試劑盒
Human growth hormone releasing peptide-Ghrelin,GHRP-Ghrelin ELISA試劑盒 
Human calmodulin,CAM ELISA試劑盒
Human true insulin,TI ELISA試劑盒
Human Noradrenaline Bitartas,NE-B ELISA試劑盒
Human prostacycline,PGI ELISA試劑盒
Human Procalcitonin,PCT ELISA試劑盒
Human ReverseTri-iodothyronine,rT3 ELISA試劑盒
Human 6-keto-prostaglandin F1a,6-K-PGF1a ELISA試劑盒
Human Pentosidine ELISA試劑盒
Human metanephrine,MN ELISA試劑盒
Human Dopamine D1 receptor,D1R ELISA試劑盒
Human adrenergic alpha-1A receptor,ADRA1A ELISA試劑盒
Human Presenilin 2,PS-2 ELISA試劑盒
Human Isoprenaline Hydrochloride,iso-Hyd ELISA試劑盒
Human Pancreastatin ELISA試劑盒
Human androgen receptor,AR ELISA試劑盒
Human Relaxin,RLN ELISA試劑盒
Human Growth Hormone Binding Protein,GHBP ELISA試劑盒
Human adrenomedullin,ADM ELISA試劑盒
Human Immunoreactive growth hormone,irGH ELISA試劑盒
Human melatonin sulfate,MS ELISA試劑盒
Rat Complement fragment 5a,C5a ELISA Kit
Rat Complement fragment 3a,C3a ELISA Kit
Rat Follistatin,FS ELISA Kit
Rat Histone Deacetylase,HD ELISA Kit 
Rat E-Selectin ELISA kit 
Rat Methylase ELISA Kit
Rat proliferating cell nuclear antigen antibody,PCNA ELISA Kit
Rat lipoprotein lipase,LPL ELISA Kit
Rat tartrate-resistant acid phosphatase 5b,TRACP-5b ELISA Kit
Rat Glucose-dependent insulin-releasing polypeptide,GIP ELISA Kit
Rat Glycogen phosphorylase II,GP-II ELISA Kit
Rat Glycogen phosphorylase MM,GP-MM ELISA Kit
Rat Glycogen phosphorylase BB,GP-BB ELISA Kit
Rat Thyroxine antibody,TAb ELISA Kit
Rat thyroid stimulating hormone receptor antidoby,TRAb ELISA Kit
Rat catecholamine,CA ELISA Kit
Rat Interleukin 27,IL-27 ELISA Kit
Rat Interleukin 23,IL-23 ELISA Kit 
Rat anti-alpha-Fodrin IgGIgA,α-Fodrin IgGIgA ELISA Kit 
Rat lipoprotein α,Lp-α ELISA Kit
Rat coagulation factor Ⅷ related antigen,FⅧ-Ag ELISA Kit
Rat Fcoagulation factor Ⅸ,FⅨ ELISA KitRat Fcoagulation factor Ⅸ,FⅨ ELISA Kit
Rat coagulation factor Ⅹ,FⅩ ELISA Kit
Rat von Willebrand Factor,vWF ELISA Kit
Rat plasmin-antiplasmin complex,PAP ELISA Kit
Rat thrombin-antithrombin complex,TAT ELISA Kit
Rat anti-thrombin receptor,ATR ELISA Kit
Rat thrombin receptor,TR ELISA Kit
Rat cardiac troponin Ⅰ,cTn-Ⅰ ELISA Kit
Rat Phosphotylinosital 3 kinase,PI3K ELISA Kit
Rat protein kinase B,PKB ELISA Kit
Rat heme oxygenase 2,HO-2 ELISA Kit
Rat Crosslaps,Cr ELISA Kit
Rat cross-linked C-opeptides of Type Ⅰ collagen,CⅠCP ELISA Kit
Rat deoxypyridinoline,DPD ELISA Kit 
Rat pyridinium crosslinkPyriLinks,PY ELISA Kit
Rat Osteopontin,OPN ELISA Kit
Rat Bone-specific Alkphase B,ALP-B ELISA Kit
Rat transferrin receptor,TFR ELISA Kit
Rat Cystatin C,Cys-C ELISA Kit
Rat Factor Ⅷ-related Antigen,FⅧAg ELISA Kit
Rat Pyruvate Kinase,M2-PK ELISA Kit
Rat acetylcholine receptor antibody,AChRab ELISA Kit
Rat substance P receptor,SP-R ELISA Kit
Rat Substance P,SP ELISA Kit
Rat alpha-L-fucosidase,AFU ELISA Kit
Rat Thyroid-Peroxidase,TPO ELISA Kit
Rat Proinsulin,PI ELISA Kit
Rat anti-IgE receptor antibody ELISA Kit
Rat anti-IVIgG antibody ELISA Kit
Rat p53tumor protein,p53TP53 ELISA Kit
Rat nitric oxide synthase,NOS ELISA Kit
Rat inducible nitric oxide synthase,iNOS ELISA KIT
Rat Endothelial nitric oxide synthase,eNOS ELISA Kit
Rat lipoprotein-associated phospholipase A2,Lp-PL-A2 ELISA Kit
Rat heat shock protein 90,hSP-90 ELISA Kit
Rat heat shock protein 70,hSP-70 ELISA Kit
Rat heat shock protein 40,hSP-40 ELISA Kit
Rat heat shock protein 20,hSP-20 ELISA Kit
Rat Cyclin-D3 ELISA Kit
Rat Cyclin-D2 ELISA Kit
Rat Cyclin-D1 ELISA Kit
Rat myelin basic protein,MBP ELISA Kit
Rat Alpha-fetoprotein,AFP ELISA Kit
Rat Prostatic Acid Phosphatase,PAP ELISA Kit
Rat Free Prostate Specific Antigen,fPSA ELISA Kit
Rat prostate specific antigen,PSA ELISA Kit
Rat ovarian cancer marker-CA125 ELISA Kit
Rat mammary carcinoma Marker-CA153 ELISA Kit
Rat Gastrointestinalcancer Marker-CA199 ELISA Kit