齊一生物膜輔蛋白（MCPCD46）檢測試劑盒英文名稱Human membrane cofactor protein,MCP ELISA Kit齊一生物科技（上海）有限公司新品*歡迎您訪問我們的如有什么問題請給我們或者留言741653262,告訴我您的需要,并留下您寶貴的意見和建議

齊一生物膜輔蛋白（MCPCD46）檢測試劑盒elisa技術流程
ELISA的基礎是抗原或抗體的固相化及抗原或抗體的酶標記.結合在固相載體表面的抗原或抗體仍保持其免疫學活性,酶標記的抗原或抗體既保留其免疫學活性,又保留酶的活性.在測定時,受檢標本（測定其中的抗體或抗原）與固相載體表面的抗原或抗體起反應.用洗滌的方法使固相載體上形成的抗原抗體復合物與液體中的其他物質分開.再加入酶標記的抗原或抗體,也通過反應而結合在固相載體上.此時固相上的酶量與標本中受檢物質的量呈一定的比例.加入酶反應的底物后,底物被酶催化成為有色產物,產物的量與標本中受檢物質的量直接相關,故可根據呈色的深淺進行定性或定量分析.由于酶的催化效率很高,間接地放大了免疫反應的結果,使測定方法達到很高的敏感度.ELISA可用于測定抗原,也可用于測定抗體.
然而,影響Elisa試驗結果的因素很多,故加強各個環節的質量保證才能充分發揮其方法學的優點.
試驗原理
試劑盒是固相夾心法酶聯免疫吸附實驗（ELISA）.已知濃度的標準品、未知濃度的樣品加入微孔酶標板內進行檢測.先將標記的抗體同時溫育.洗滌后,加入親和素標記過的HRP.再經過溫育和洗滌,去除未結合的酶結合物,然后加入底物A、B,和酶結合物同時作用.產生顏色.顏色的深淺和樣品中的濃度呈比例關系.
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