齊一生物供應(yīng)清道夫受體B（SRBCD36）檢測(cè)試劑盒英文名稱Human scavenger receptor B,SRB ELISA Kit齊一生物科技（上海）有限公司新品*歡迎您訪問我們的如有什么問題請(qǐng)給我們或者留言741653262,告訴我您的需要,并留下您寶貴的意見和建議

齊一生物供應(yīng)清道夫受體B（SRBCD36）檢測(cè)試劑盒elisa技術(shù)流程
ELISA的基礎(chǔ)是抗原或抗體的固相化及抗原或抗體的酶標(biāo)記.結(jié)合在固相載體表面的抗原或抗體仍保持其免疫學(xué)活性,酶標(biāo)記的抗原或抗體既保留其免疫學(xué)活性,又保留酶的活性.在測(cè)定時(shí),受檢標(biāo)本（測(cè)定其中的抗體或抗原）與固相載體表面的抗原或抗體起反應(yīng).用洗滌的方法使固相載體上形成的抗原抗體復(fù)合物與液體中的其他物質(zhì)分開.再加入酶標(biāo)記的抗原或抗體,也通過反應(yīng)而結(jié)合在固相載體上.此時(shí)固相上的酶量與標(biāo)本中受檢物質(zhì)的量呈一定的比例.加入酶反應(yīng)的底物后,底物被酶催化成為有色產(chǎn)物,產(chǎn)物的量與標(biāo)本中受檢物質(zhì)的量直接相關(guān),故可根據(jù)呈色的深淺進(jìn)行定性或定量分析.由于酶的催化效率很高,間接地放大了免疫反應(yīng)的結(jié)果,使測(cè)定方法達(dá)到很高的敏感度.ELISA可用于測(cè)定抗原,也可用于測(cè)定抗體.
然而,影響Elisa試驗(yàn)結(jié)果的因素很多,故加強(qiáng)各個(gè)環(huán)節(jié)的質(zhì)量保證才能充分發(fā)揮其方法學(xué)的優(yōu)點(diǎn).
試驗(yàn)原理
試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè).先將標(biāo)記的抗體同時(shí)溫育.洗滌后,加入親和素標(biāo)記過的HRP.再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.
MA183971    Factor H    100 μg
MA183977    Alix    100 μg
MA183978    CD18 (FITC)    100 μg
MA183987    Complement C3d    100 μg
MA1840    SRC1    100 μl
MA184003    ACTH    200 μg
MA184005    FOXP1    100 μg
MA184008    Pierce(R) Anti-CD16 MonoclonalMouse Antibody, 200 ug    200 μg
MA184018    CD8 alpha    250 μg
MA184019    CD3    250 μg
MA184031    MHC II HLA-DP/DQ/DR    200 μg
MA1842    Ncor2    100 μg
MA1843    Ncor2    100 μg
MA1845    SRC3    100 μg
MA1846    PRC1    100 UG
MA1847    Nup358    100 μg
MA1848    PLK1    100 μg
MA1850    gamma Tubulin    100 μg
MA1860    ErbB3    200 μg
MA1861    ErbB4    200 μg
MA1870    RPA 34    100 μl
MA1872    AP-2    100 μl
MA190001    HEF1    50 μl
MA190008    PGP 9.5    500 μl
MA190031    CD66a, CD66c + CD66d    100 μg
MA190035    Rat IgG1 (Isotype Control)    100 tests
MA190039    CA125    100 μg
MA190041    Lamin B1 + B2    1 ml
MA190048    PI 3 Kinase p85 beta    50 μg
MA190055    beta 2 Microglobulin    200 μg
MA190059    ALK-80    6 ml
MA190073    Bcl-rambo    50 μg
MA190087    RAF1    50 μg
MA190088    TCP1 beta    50 μg
MA190102    CD172a (Biotin)    100 μg
MA190103    CD172a (FITC)    100 μg
MA190116    MAdCAM1    250 μg
MA190154    v-Myb + c-Myb    100 μg
MA190174    EpCAM    5 ml
MA190180    IgM    500 μg
MA190181    IgM    1 mg
MA190184    AChR alpha subunit    100 μl
MA190186    FK506    100 μg
MA190193    URO8    500 μl
MA190203    CDw78    200 tests
MA190218    MLH1    50 μg
MA190222    AChR gamma subunit    500 μl
MA190231    CD249DCE    200 μg
MA190233    CD42a    500 μl
MA190234    Ki1    500 μl
MA190252    Myelin basic protein    500 μl
MA190265    AP2 alpha + beta    100 μg
MA190266    AP2 Transcription Factor    1 ml
MA190268    IgG CH3 domain    100 μg
MA190302    Factor XII Heavy chain    100 μg
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