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人脂肪細胞型脂肪酸結合蛋白(FABP4) ELISA Kit
人細胞因子信號轉導抑制因子3(SOCS3) ELISA Kit
現貨血紅蛋白(Hb)檢測試劑盒(血清.血漿.組織)價格:全國:或咨詢在線客服,或者以郵件形式發到我司741653262,齊一生物科技(上海)有限公司提供大量品牌進口原裝,分裝,以及穩定性強的國產ELISA試劑盒,性價比*。
現貨血紅蛋白(Hb)檢測試劑盒(血清.血漿.組織)ELISA試劑盒操作步驟
ELISA試劑盒使用前,將所有試劑充分混勻.不要使液體產生大量的泡沫,以免加樣時加入大量的氣泡,產生加樣上的誤差.
根據待測樣品數量加上標準品的數量決定所需的板條數.每個標準品和空白孔建議做復孔.每個樣品根據自己的數量來定,能使用復孔的盡量做復孔.標本用標本稀釋液1:1稀釋后加入50ul于反應孔內.
加入稀釋好后的標準品50ul于反應孔、加入待測樣品50ul于反應孔內.立即加入50ul的標記的抗體.蓋上膜板,輕輕振蕩混勻,37℃溫育1小時.
甩去孔內液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復此操作3次.如果用洗板機洗滌,洗滌次數增加一次.
每孔加入80ul的親和鏈酶素-HRP,輕輕振蕩混勻,37℃溫育30分鐘.
甩去孔內液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復此操作3次.如果用洗板機洗滌,洗滌次數增加一次.
每孔加入底物A、B各50ul,輕輕振蕩混勻,37℃溫育10分鐘.避免光照.
取出酶標板,迅速加入50ul終止液,加入終止液后應立即測定結果.
在450nm波長處測定各孔的OD值.
ELISA操作步驟
酶聯免疫吸附實驗材料,試劑,溶液
1.酶標板,100μltip頭,1mlEp管,濕盒
2.包被稀釋液(0.05mol/L碳酸鈉-碳酸氫鈉buffer,pH9.6)
碳酸鈉0.15g,碳酸氫鈉0.29g,疊氮鈉0.02g,加雙蒸水至100ml,調至pH9.6
3.封閉液(5%小牛血清/PBS溶液)
小牛血清5ml,1*PBS(pH7.4)95ml
4.洗滌液(PBST,pH7.4)
NaCl0.8g,KH2PO40.02g,Na2HPO4.12H2O0.29g,KCl0.02g,Tween200.05ml,疊氮鈉0.01g,加雙蒸水至100ml,調至pH7.4
5.樣本稀釋液(PBS,pH7.4)
NaCl0.8g,KH2PO40.02g,Na2HPO4.12H2O0.29g,KCl0.02g,疊氮鈉0.01g,加雙蒸水至100ml,調至pH7.4
6.酶標第2抗體(羊抗兔)稀釋范圍1:5,000-1:100,000
7.底物液(TMB-過氧化氫尿素溶液)
①底物液A:TMB20mg,無水乙醇10ml,加雙蒸水至100ml.
②底物液B(0.1mol/L檸檬酸-0.2mol/L磷酸二氫鈉緩沖液,pH5.0-5.4)
Na2HPO41.46g,檸檬酸0.933g,0.75%過氧化氫尿素0.64ml,加三蒸水至100ml,調至pH5.0-5.4
③底物A和B按1:1混合即成TMB-過氧化氫尿素溶液.
8.終止液(2mol/LH2SO4溶液)雙蒸水200ml,濃硫酸34ml,(緩慢滴加并不斷攪拌),加雙蒸水至300ml9.0.9%生理鹽水
IGF2 50 μg
Growth Hormone, Human 500 μg
Leptin 1 mg
EGF 500 μg
FGF basic 50 μg
BDNF 10 μg
Keratinocyte Growth Factor 10 μg
Keratinocyte Growth Factor-2 25 μg
VEGF 10 μg
TNF alpha 50 μg
MIP-1 alpha 20 μg
FGF acidic 20 μg
IL-1 Receptor Antagonist 10 μg
Parathyroid Hormone 100 μg
EGF 1000 μg
Growth Hormone, Human 1000 μg
IGF1 1000 μg
KGF 20 μg
Insulin 250 mg
Leptin 5 mg
Parathyroid Hormone 500 μg
PRL-1 10 μg
PRL-2 10 μg
PTP1B 20 μg
TC-PTP 20 μg
MEG-1 20 μg
MEG-2 20 μg
血紅蛋白(Hb)檢測試劑盒SHP-2 20 μg
PEST 20 μg
PTP beta 20 μg
RPTP gamma 20 μg
Sphingosine Kinase 1 10 μg
Sphingosine Kinase 2 10 μg
Sphingosine Kinase 2, Long 10 μg
Sphingosine Kinase 1a 10 μg
Estrogen Receptor alpha 140 UG
Estrogen Receptor beta 180 UG
ALK/p80 10 μg
Cadherin E 10 μg
Calretinin 10 μg
Cathepsin D 10 μg
CD2 10 μg
CD23 10 μg
CD34 10 μg
CD4 10 μg
CD68 10 μg
CD99 10 μg
c-erbB2 10 μg
Chromogranin A 10 μg
D4GDI 10 μg
FHIT 10 μg
Gp100 10 μg
IGF1 Receptor 10 μg
Involucrin 10 μg
Keratin 8 10 μg
LYVE-1 10 μg
MAGE-1 10 μg
NY-ESO-1 10 μg
p53 mutant 10 μg
p53 wild type 10 μg
PCNA 10 μg
PGP9.5 10 μg
PgR 100 UL
PPAR gamma 10 μg
PTEN 10 μg
S100 beta 10 μg
S100A4 10 μg
SOX9 10 μg
TGF beta 1 10 μg
Factor VII (human) 20 μg
Factor IX (human) 100 μg
Factor IX (bovine) 100 μg
9016537 Transportation box, BioSprint 96
9016463 Vacuum Tray, complete, BR8 RD, UK
9016267 BioRobot MDx Performance Upgrade Kit
9019184 Temp. Sensor, PyroQ96
9019178 Opto - plate lock open, PyroQ96MD
9019879 Label, cover, main, RG6
9019876 Cover, main, red, RG6
9019762 Fuse, 4A, 250VAC, V2, QX
9019733 Motor, Z, V2, QX
9019726 Transport lock, V2, QX
9013404 Cable set, cooling system, 200
9238803 Rect. sealing cap, clear, incl. clips
9019653 Base Plate VPT, PyroQ96VW
9019626 Tip ejector, Cx2
9019603 Pins, locating, Mix and Reagent set, CG1
9019598 Lead, vac trap sensor, CX1
9019573 Belt, X-axis assembly, CQx
9019546 Clamp, cable, 3 wires 2 tabs, Cxx
9019533 PCB, USB, QGY
9019518 RISER VACUUM 16.25MM
217004 miRNeasy Mini Kit (50)
204243 QuantiTect SYBR Green RT-PCR Kit (200) For 200 x 50 ul reactions: 3 x 1.7 ml QuantiTect SYBR Green RT-PCR Master Mix,* 100 ul QuantiTect RT Mix, 2 x 2.0 ml RNase-free water
9019439 Heating block assy
9019425 Gasket, lower chamber, RG3
9019416 Box, shipping with foam packing, RG3
9019401 Connector, COM incl. int. cable, RG3
9019360 Switch, mains voltage selector, RG6
1027417 Flexitube siRNA 5 nmol
9019339 LED, green (530), RG6
9019331 EPROM, IC Motherbrd, state FW, RG6
9019237 Linear guide - X, PyroQ24
9234673 X-Assembly Turntable Card
9234659 Cassette, HSPS, click'n'go
1026956 Buffer AVE (Elution Buffer) (125/125),KG
1026905 RNeasy 96 Plates (12)
9233674 Probe, ceramic coat., 0.4mm, 158mm
9233630 Mousepad BR 01/10 (WW)
9019209 Motor - Gripper, PyroQ96MD
9019197 Damper kit - Robot, PyroQ96MD
1020955 Buffer AW2 with Azid (127/500),KG
1062791 HBV PCR REAGENT III
9232903 Spritzenpumpe mMBP 2021C P7xxx
1061065 PG Influenza A RT-PCR Assay (48) Z
9232900 Valve, release, auto vac, BR8000
9016710 Software Package LiquiChip Analyzer
9019891 Pipetting head, 8 ch. , CX1
9019881 Lid with LED, red, RG6
9019857 36-well High Profile Locking Ring
9019837 IPC Hard Drive PCA6751, PyroQ96MD
9022832 Notebook_V3 W7_32bit
9013703 Tubing, syringe to connector, BR3000
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