Super Script Ⅱ陰性逆轉錄酶廠商

級別：BR 

來源：Purified from E. coli expressing the pol gene of M-MLV (1,2), mutagenized to reduce the RNase H activity
濃度：200u/ul 

Super Script Ⅱ陰性逆轉錄酶廠商

活力定義：One unit of SuperScript® II is the amount of enzyme required to incorporate 1 nmole of eoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)•oligo(dT)12-18 as template•primer (3).

Unit Reaction Conditions：50 mM Tris-HC1 (pH 8.3), 40 mM KC1, 6 mM MgC1 2 , 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18 , 0.1 mg/m1 BSA, and enzyme in 50 µl for 10 min. at 37℃

Performance and Quality Testing：SDS-PAGE purity,endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays, and yield and length of cDNA product

Enzyme Function：RNA dependent DNA Polymerase

Final Product Size：12.3 kb or less

Number of Reactions：10 Reactions

Optimal Reaction Temperature：42℃

Sensitivity：Medium

Template：ssRNA,ssDNA,RNA-DNA Hybrid

Super Script Ⅱ陰性逆轉錄酶廠商

產品描述：SuperScript® II Reverse Transcriptase (RT) is an improved version of SuperScript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations rather than a deletion in the RNase H active center. Like SuperScript® RT, SuperScript® II RT has reduced RNase H activity. Unlike SuperScript® RT, however, the selective mutations within the RNase H domain maintain full polymerase activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript® II RT superior performance characteristics, including：Greater first-strand cDNA yields,More full-length cDNA synthesis,Full activity at 42℃
性狀：懸浮液
用途：生化研究。
保存：-20℃