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產品名稱:豬熱休克蛋白20(HSP-20)ELISA Kit
英文名稱:Porcine Heat Shock Protein 20,HSP-20 ELISA Kit
豬熱休克蛋白20(HSP-20)ELISA Kit |
豬熱休克蛋白20(HSP-20)ELISA Kit |
豬熱休克蛋白20(HSP-20)ELISA Kit |
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標本的采集及保存
1.血清:全血標本請于室溫放置2小時或4℃過夜后于1000g離心20分鐘,取上清即可檢測,或將標本放于-20℃或-80℃保存,但應避免反復凍融。
2.血漿:可用EDTA或肝素作為抗凝劑,標本采集后30分鐘內于2 - 8°C 1000 g離心15分鐘,或將標本放于-20℃或-80℃保存,但應避免反復凍融。
3.細胞培養物上清或其它生物標本:1000g離心20分鐘,取上清即可檢測,或將標本放于-20℃或-80℃保存,但應避免反復凍融。
檢測方法:ELISA酶聯免疫(雙抗夾心法)
檢測標本:血清、血漿、組織、細胞上清液、尿液等。
ELISA試劑盒的組成:
1.30倍濃縮洗滌液 20ml×1瓶 7.顯色劑B液 6ml×1/瓶
2.酶標試劑 6ml×1瓶 8.標準品(96μg/L) 0.5ml×1瓶
3.酶標包被板 12孔×8條 9.標準品稀釋液 1.5ml×1瓶
4.樣品稀釋液 6ml×1瓶 10.說明書 1份
5.顯色劑A液 6ml×1瓶 11.封板膜 2張
6.顯色劑B液 6ml×1/瓶 12.密封袋 1個
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