北京諾博萊德科技有限公司供應pET-32a(+) NobleRyder D0064；現貨供應pET-32a(+) NobleRyder D0064量大優惠，咨詢 ：        1215878164
 

載體類型：大腸桿菌表達載體
載體大小： 5900 bp
載體宿主：大腸桿菌（E.coli）
細菌抗性：氨芐*（Amp）
5' 測序引物：S Tag primer
3' 測序引物：T7 terminator primer
載體簡介：
The pET-32 series is designed for cloning and high-level expression of peptide sequences fusedwith the 109aa Trx Tag thioredoxin protein . Cloning sites are available for producing fusionproteins also containing cleavable His Tag and S Tag sequences for detection and puri cation.Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression regionof the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented
so that infection with helper phage will produce virions containing single-stranded DNA that corre-sponds to the coding strand. Therefore, single-stranded sequencing should be performed using theT7 terminator primer.

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